Names: Key Hour: Date: /25 Points - Menifee County
Names: Key
Hour:
Date:
/25 Points
As a group of four, decide who is going to carry out which job (Jobs can be found on page 2). Follow the instructions below for the three numbers assigned to your group from the sentence code sheet (Page 3). Be sure to fill out the blanks on both pages 1 and 2 as you go.
1. The DNA stays in the nucleus (which is your assigned lab table). The DNA has the sentence code sheet out focusing on the # assigned to your lab group. This person's job is to actually read out loud the letter code from the code sheet. Remember: each sentence code represents one side of a DNA molecule. (3 Points)
#
#
#
2. The m-RNA transcribes each codon from the DNA, using base pairing rules, onto the lines below. This process is transcription; which is the first step of protein synthesis. (remember to use U instead of T) This step occurs in the nucleus. Example: DNA says "CCC" you write "GGG". (3 Points)
#
code:
#
code:
#
code:
3. The m-RNA leaves the nucleus with the transcribed code and goes to the cytoplasm (the rest of the classroom) to find the ribosome (the student desks). (1 point)
4. The m-RNA and the ribosome tell the t-RNA which anti-codons are needed (cards around
the room). The ribosome writes the anti-codon instructions on the lines below for t-RNA to
take when searching for the appropriate anti-codon and the corresponding amino acid. (3
Points)
#
anti-codon:
#
anti-codon:
#
anti-codon:
5. The t-RNA searches the cell (classroom) for the correct amino acid (word on the back of the anti-codon card) and begins to create the protein (sentence). This completes the translation step of protein synthesis. The t-RNA should write the sentence on the lines below. (3 Points)
#
protein:
#
protein:
#
protein:
LZHS ? Chapter 12 Protein Synthesis Lab
1
Biology I CP
You are responsible for taking notes on each role. You must include information such as where each person performed their function, what their function was, which step of protein synthesis they were involved in, which types of nucleotides were involved, how many strands were involved, etc...
Person #1 = DNA (Name:
)
Where function performed (cytoplasm or nucleus) nucleus
What is their function stores information (read code)
Which step of protein synthesis was involved (transcription or translation)
Which nucleotides were involved (ATCG or AUCG) ATCG
How many strands were involved (one or two)
2
Other Important Info.
transcription
Person #2 = m-RNA (
Name:
)
Where function performed nucleus & cytoplasm
What is their function transcribes each codon from DNA
Which step of protein synthesis was involved
transcription
Which nucleotides were involved AUCG
How many strands were involved 1
Other Important Info.
Person #3 = r-RNA (Name:
)
Where function performed ribosome
What is their function
tell tRNA which anticodons are needed
Which step of protein synthesis was involved
transcription
Which nucleotides were involved AUCG
How many strands were involved 1
Other Important Info.
Person #4 = t-RNA (Name:
)
Where function performed cytoplasm
What is their function
find correct amino acid & begins to create protein
Which step of protein synthesis was involved
translation
Which nucleotides were involved AUCG
How many strands were involved 1
Other Important Info.
LZHS ? Chapter 12 Protein Synthesis Lab
2
Biology I CP
Sentence Code Sheet
Each "sentence code" below represents one side of a DNA strand. You must imagine that you have "unzipped" a DNA strand and are now focusing on only one side. You will be transcribing the code into an m-RNA strand and further translating the code using a tRNA molecule with an appropriate amino acid attached. You will, however, be creating a sentence using words, rather than a protein using amino acids. Good Luck!
1. ATGAAAAACAAGGTACACATCTAG 2. ATGAAAAACAATTGCACGTAG 3. ATGTAAACCACTACATAG 4. ATGAGAAGTAGGAGAAGCATAATCTAG 5. ATGATTCAACACATCCAGCCACATTAG 6. ATGCCCCCGAGAAGCCCTTAG 7. ATGCGACGCCGGCGTTAG 8. ATGCTACTCATAGATCTGCTTTAG 9. ATGTAAAGGGAAGACGAGTAG 10. ATGCCCCCGGCAGCCGCGTAG 11. ATGGCTCCGAGAGGAGGCAGAGGGTAG 12. ATGAAAGGTAAGGTAGTCTAG 13. ATGAAAGTGAAGGTTTAG 14. ATGTAAAGGGAATACTATTCATAG 15. ATGTAATCCTCGTCTCGGCGTTAG 16. ATGATAGATCTGCTTCCGAGAAGCTAG 17. ATGCCCCCGGAATGATGCTAG 18. ATGTGGGTATGTCGGCGTTAG 19. ATGTTACCGAGATTCTTGTTTTAG
20. ATGTTATCCTCGTGGTTGTTTTAG
LZHS ? Chapter 12 Protein Synthesis Lab
3
Biology I CP
AAC UAA CGA GUA UAU CGG GUG GAC AAU UCG CCG UGC UGG
MOTHER WE
DRINK A
THIS EVERY BROTHER DEMENTED DRESSES
BE IS YOU READ
LZHS ? Chapter 12 Protein Synthesis Lab
4
Biology I CP
CGU UCU UUG GUC GAA UUA AGU CGC AUU CAA CUG GCU AGA
DAY INFORMED
FOR DRESS
ALL DNA BEATLES WATER AN OLD ROLL EDUCATION THE
LZHS ? Chapter 12 Protein Synthesis Lab
5
Biology I CP
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