BIOLOGY 105: Study Questions for Exam 2 (Sebastian Kadener)

A protein extract from the nuclei of cultured frog cells was passed through a DEAE Sephadex column to which charged proteins absorb differentially. Adsorbed proteins were eluted (black curve) with a solution of constantly increasing NaCl concentration. Fractions containing the eluted proteins were assayed for the ability to transcribe DNA (red curve) in the presence of the four ribonucleoside ... ................
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