The Inoue Method for Preparation and Transformation of ...
Per Liter: To 950 ml of deionized H2O , add: Tryptone 10 g Yeast Extract 5 g NaCl 10 g Shake until the solutes have dissolved. Adjust the pH to 7.0 with 5 N NaOH (~0.2 ml). Adjust the volume of the solution to 1 liter with deionized H2O . Sterilize by autoclaving for 20 minutes at 15 psi (1.05 kg/cm2) on liquid cycle. SOB Medium. Per Liter: ................
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