Gene Expression Profiling using Quantitative Polymerase ...
Primer design: Primers were designed using the Integrated DNA Technology (IDT) “Primer Quest Tool.” All mus musculus gene sequences were searched for on the “National Center for Biotechnology Information” ‘PubMed’ website, under the nucleotide section. All primers designed had a GC content between 45-55%, to ensure proper annealing. ................
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