Section 1: - Michigan State University



DATE: March 27, 2020POLICY NUMBER: EHS-002CRevision #3.0(3.2020)SOP TITLE: OSMIUM TETROXIDEAUTHOR(S): APPROVED By:STANDARD OPERATING PROCEDURE OSMIUM TETROXIDEResearch Group:_____________________________________________________________Author:_____________________________________________________________________Last revision date: ____________________________________________________________Room and Building:___________________________________________________________Contact information: __________________________________________________________Section 1: This standard operating procedure is for□ The general use of osmium tetroxide □ A specific laboratory procedure involving osmium tetroxide: _____________________________Section 2: Physical HazardsSynonyms: Osmic acid, perosmic acid, osmium (VIII) oxide, OsO4CAS Number: 20816-12-0Osmium tetroxide is a highly volatile, highly toxic chemical that is used in electron microscopy staining and as a biological fixative.Permissible exposure limits: MIOSHA (Michigan Occupational Safety & Health Administration) TWA (Time-Weighted Average)=0.002 mg/m3; MIOSHA STEL (Short-Term Exposure Limit)=0.006 mg/m3Section 3: Potential HazardsOsmium tetroxide is a strong oxidizer that will sublime readily at room temperature. Contact with combustible materials may cause fire. Reaction with hydrochloric acid will produce chlorine gas.Osmium tetroxide is acutely toxic and is a severe irritant to the eyes and respiratory tract. Osmium tetroxide vapor can cause staining to the cornea at low levels of exposure. Initial symptoms of eye exposure can include lacrimation and grittiness in the eye and may also include the appearance of rings and/or cloudiness of the vision. Cumulative exposure can result in delayed onset of symptoms several hours after exposure.Inhalation of osmium tetroxide vapors can cause coughing, difficulty breathing and may be fatal. Skin contact can cause dermatitis, severe irritation or chemical burns.Chronic exposure to osmium tetroxide can result in accumulation of osmium compounds in the liver and kidney. It has been reported as a reproductive toxin in animals. Small, chronic exposures to osmium tetroxide via inhalation, skin contact or eye contact can lead to systemic health effects over time.Section 4: Personal Protective EquipmentThe following is the minimum personal protective equipment (PPE) when working with osmium tetroxide:Chemical goggles – Safety glasses DO NOT offer adequate protection from osmium tetroxide vapors.Nitrile gloves – Double gloves when working with pure or highly concentrated solutions. DO NOT use latex gloves with any concentration of this osmium tetroxide. Wash hands after removing gloves. Change gloves often and when punctured or torn.Laboratory coat – If the lab coat becomes contaminated with OsO4, remove, place in fume hood and decontaminate spill area with corn oil. Double-bag laboratory coat and send to MSU EHS for disposal.State where PPE for this procedure can be found in the laboratorySection 5: Engineering ControlsOsmium tetroxide must be handled in a certified fume hood, never on an open bench. Biosafety hoods do not offer adequate protection. Keep hood clear of flammable/combustible materials. Keep baffles clear of debris. Ensure fume hood is in working order before beginning work. Keep sash as low as possible without impeding work processes. Keep away from hydrochloric acid, other acids, bases, organic materials, metals, strong reducing agents and strong oxidizing agents.Describe engineering controls that will be used to minimize potential exposure to osmium tetroxide, such as how the experiment will be contained in a fume hood or glove box.Describe how sources of ignition, incompatible chemicals and/or combustible materials will be controlled in the work area. List locations of functioning safety showers and emergency eyewashes.Section 6: Special Handling and Storage RequirementsOsmium tetroxide vapors can penetrate plastics. Store in a sealed glass container with unbreakable secondary containment. Refrigeration can reduce sublimation; however, this product should not be stored with other chemicals inside of it. A small refrigerator dedicated to osmium tetroxide storage can be used.Osmium tetroxide is a minimum two-person procedure. No working alone with osmium tetroxide is permitted. Osmium tetroxide is not to be used by any undergraduate students in any form.Describe how osmium will be secured and stored within the laboratory.Describe the handling procedures for osmium tetroxide inside and outside of fume hood.List anticipated purchase amounts, and maximum amounts of osmium tetroxide expected to be in the laboratory at any time.Section 7: Accidental Release ProceduresSmall spills inside fume hood (< 2 mL):Ensure appropriate PPE is worn (see #4 above)Cover the spill with corn oil. Oil will turn black when it reacts with the OsO4Absorb the corn oil/OsO4 solution with absorbent from spill kit.Scoop up and place in doubled plastic bag. Attach appropriate waste tag and submit waste request online.Clean area with detergent solution to remove residual corn oil.Larger spills (> 2 mL) and spills outside of the fume hood:Alert other personnel in the immediate areaEvacuate area, closing all doors to the laboratoryTelephone MSU EHS at 355-0153 for assistance, or 355-2222 after hoursTelephone 911 if an injury or fire has occurred as a result of the spillKeep others from entering area until MSU EHS confirms area is safe for occupancyChemical exposureIf vapor has been inhaled, move victim to fresh air immediatelyIf osmium tetroxide has been splashed on skin or in eyes, begin rinsing affected area with copious amounts of waterTelephone 911 immediately and request medical assistance Give MSDS to emergency personnelSection 8: Exposure ProceduresSkin exposure: Call 911 immediately for assistance. Rinse affected skin with plenty of water while removing contaminated clothing and shoes. Rinse for at least 15 minutes or until help arrives.Eye exposure: Call 911 immediately. Wash eyes for at least 15 minutes or until help arrives, lifting the upper and lower eyelids.Inhalation exposure: Call 911 immediately. Move to uncontaminated environment and wait for help to arrive.Section 9: Waste Disposal ProceduresAll osmium tetroxide liquid waste, solid waste, storage containers, and contaminated debris must be disposed of by submitting online waste pickup requests () every 90 days to MSU EHS. Double bag contaminated debris. Keep osmium tetroxide waste in the fume hood until waste pickup.Use the following decontamination methods before sending osmium tetroxide to MSU EHS disposal:Osmium tetroxide powder and solution should be combined with corn oil before disposal. Ensure compound is neutralized by placing a piece of filter paper soaked in corn oil above the solution. If the filter paper turns black, the solution has not been effectively neutralized and more corn oil should be added to the solution.Osmium tetroxide solutions that cannot be placed in corn oil must be sent for waste disposal via EHS in a glass container with a shatterproof secondary container in place.Section 10: Material Safety Data Sheets / Safety Data SheetsList location(s) of (M)SDS for the osmium tetroxide. This should include a printed copy in the laboratory in an easily accessible location.Section 11: Training and AwarenessEmployees working with acids must complete the following training:□ Chemical Hygiene and Hazardous Waste Initial / Refresher□ Site Specific Training with PI or lab manager□ Review and signature of this completed SOP□ Other ________________________________________________________________If appropriate, identify other employees in the vicinity who may be affected should an unintended reaction or release of acid occur. Describe how those employees will be notified of this experiment, the location of this SOP and (M)SDS, and point contact person available for questions.Section 12: ProtocolsAttach a copy of your protocol here, including an estimate of how long the process takes and how frequently it will be conducted. Provide a general sequential description of work, including details such as amounts of osmium tetroxide used, special safety equipment utilized, personal protective equipment required for each procedure, waste disposal procedures, etc. as appropriate. Section 13: SOP Review and Prior ApprovalI, the PI/Supervisor, grant the following laboratory personnel approval to perform the above SOPName: _______________________________________________________________________________Name: _______________________________________________________________________________Name: _______________________________________________________________________________PI/Laboratory Supervisor signature: _____________________________________ Date: _____________I have reviewed and understood this Standard Operating Procedure, and agree to abide by the protocols described herein:Signature:______________________________________________________ Date:__________________Signature:_____________________________________________________ Date:__________________ Signature:_____________________________________________________ Date:__________________A completed copy of this Standard Operating Procedure has been reviewed and approved by MSU Office of Environmental Safety:MSU EHS Staff: ____________________________________________________ Date:_______________REVISION DATE:REVISION SUMMARY: REVISION COMPLETED BY: ................
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