Currituck County Schools



Biotechnology ReviewDefine “genetic engineering.”What is the purpose of gel electrophoresis?What scientists would use gel electrophoresis to identify DNA of suspects?The steps of gel electrophoresis are scrambled below. Place them in the correct order in the chart below. Fragments are run on the gel through an electric current; DNA samples are cut into different sizes using restriction enzymes; Bands can be read under a UV light or through staining; Different bands can be compared for genetic similarities; Longer fragments move slower through the gel (not far) and shorter fragments move quickly (farther)STEPS IN GEL ELECTROPHORESIS1.2.3.4.5.4505325109220211455029464085725294640Solve the problems below by interpreting the various gels. Sample “A” represents DNA evidence pulled from a crime scene. Which suspect committed the crime?Which species are most closely related? Why?A woman (1) has 2 sons from two separate marriages. Determine which father (2 and 3) matches which son (4 and 5). Define “transgenic organism.” Give 1 example of a transgenic organism used in agriculture, and a 2nd example used in pharmaceutical companies.Describe each step of bacterial transformation below: The Human Genome Project started in _____________ and ended in ____________.Describe the purpose of the Human Genome Project. What is gene therapy?Describe some ethical issues involved in gene therapy.For each box in the chart below, describe the two sides of the ethical issues related to the biotechnology practice.GMO’sCloningStem Cell Research ................
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