Supplementary Materials and Methods - AACR Journals
Supplementary Materials and Methods. ELISA analysis. To prepare samples for enzyme-linked immunosorbent assay (ELISA) analysis, NCI-H292 or A-431 cells were seeded into 96-well tissue culture plates in base medium supplemented with 10% FBS and pen/strep and allowed to grow for 24 hr, after which time serum was reduced by replacing medium with base medium supplemented with 1% FBS and pen/strep. ................
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