“EXTRACTION, PHYTOCHEMICAL STUDY, EVALUATION AND ...



PHYTOCHEMICAL INVESTIGATION AND EVALUATION FOR ANTHELMINTIC ACTIVITY OF ETHANOLIC EXTRACT OF

CINNAMOMUM TAMALA NEES & EBERM LEAVES

M.PHARM DISSERTATION PROTOCOL

SUBMITTED TO

Rajiv Gandhi University of Health Science, Karnataka Bangalore

BY

LOVHARE RAHUL BATUSING B.Pharm

Under the guidance of

Dr. G. K. KAPSE M.Pharm., Ph.D

PROFESSOR

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DEPARTMENT OF PHARMACEUTICAL CHEMISTRY

LUQMAN COLLEGE OF PHARMACY, GULBARGA-585102

2011-2012

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES, KARNATAKA, BANGALORE

ANNEXURE II

PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION

|1. |Name of the candidate |MR. lOVHARE RAHUL BATUSING |

| |And address |c/o mr.b.c.johari (postman-shahada) |

| | |NITIN NAGAR , PLOT NO.08, INFRONT OF |

| | |MAHAVIR HOSPITAL, SHAHADA |

| | |post: shahada-425409. dist: nandurbar, [MAHARASHTRA] |

|2. |Name of the institution |Luqman college of pharmacy, |

| | |old jewargi road, |

| | |gulbarga-585102. |

|3. |Course of study and subject |m.pharm |

| | |(Pharmaceutical Chemistry) |

|4. |date of adimission to course | |

| | |08 -06-2011 |

|5. |TITLE OF THE TOPIC: |PHYTOCHEMICAL INVESTIGATION AND EVALUATION FOR ANTHELMINTIC ACTIVITY OF ETHANOLIC |

| | |EXTRACT OF |

| | |CINNAMOMUM TAMALA NEES & EBERM LEAVES |

|6 |Brief Resume of the intended work: |

| |6.1 Introduction |

| |Helminthiasis or worm infestation is one of the most prevalent disease and one of the most serious public health problems in the world. Hundreds|

| |of millions if not billions of human infections by helminthes exist worldwide and with increased world travel and immigration from the |

| |developing countries1. |

| |Antihelmintics are the drugs that expel parasitic worms (helminthes) from the body by either stunning (vermifuges) or by killing (vermicides) |

| |them. For the treatment of helminthiasis, there are allopathic drugs like Albendazole, Mebendazole, Piperazine, Levamisole, Dethyl carbamazine |

| |are available. Also, there are herbs used are Artemisia absinthium, Adiantum capillus, Cedrus deodara, Nerium indicum, Cassia angustifolia etc2.|

| | |

| | |

| |6.2 Need for the study: |

| |There are different approaches for the selection of plants that may contain new biologically active compounds3. One of the approaches used is |

| |the ethno medical data approach, in which the selection of a plant is based on the prior information on the folk medicinal use of the plant. It |

| |is generally known that ethno medical data provides substantially increased chance of finding active plants relative to random approach4. |

| |One of the such finding is that reveals the use of plant Cinnamomum tamala Nees & Eberm which has been used in Indian folk medicine as tonic to |

| |brain, anthelmintic, diuretic and treating diseases of the anus and rectum5. |

| |Cinnamomum tamala Nees & Eberm belonging to the family Lauraceae, commonly known as Tejpat, is a small evergreen tree found throughout India |

| |along the North-Western Himalayas, Sikkim, Assam, Meghalaya, Central and Southern India. Plant is highly distributed sub tropical Himalaya and |

| |cultivated as spice in India6. |

| |They are habitat in tropical & subtropical Himalayas (3000-7800 ft) and in Sylhet and Khasia hills (3000-4000 ft)7. Bark of the plant is |

| |aromatic, stimulant and is also used to treat rheumatism, colic diarrhea and scorpion sting. Leaves are tonic to brain, diuretic, hypoglycemic |

| |and also used to treat respiratory tract infections5. |

| |The present study is an attempt to investigate the phytochemical composition, isolation and evaluation for the anthelmintic activity of |

| |ethanolic extract and its isolated compounds of Cinnamomum tamala Nees & Eberm leaves. |

| | |

| | |

| |The Cinnamomum tamala Nees & Eberm belongs to family Lauraceae, which has been claimed to possess anthelmintic activities also5. Literature |

| |survey reveals that a detailed chemical investigation on the leaves of Cinnamomum tamala Nees & Eberm and its evaluation for its biological |

| |activities is required which has not been explored completely yet7-11. Hence, thorough phytochemical and pharmacological studies can be carried |

| |out using this plant material. The investigations on these bioactive entities of Cinnamomum tamala Nees & Eberm leaves will yield information |

| |about chemical constituents and evaluation of the extracts and isolated compounds for the above mentioned activities that might be of great use |

| |for scientists involved in various facets of Pharmaceutical research7. |

| | |

| |6.3 Objective of the study: |

| |Identification and authentication of the plant Cinnamomum tamala Nees & Eberm. |

| |Collection and shade drying of the plant material of Cinnamomum tamala Nees & Eberm leaves. |

| |Soxhlet extraction of the leaves powder by sequential extraction with suitable solvents i.e. petroleum ether (40-450C), chloroform, ethanol |

| |(95%) followed by distilled water. |

| |Preliminary phytochemical screening to investigate the chemical composition of all the above mentioned extracts. |

| |Isolation and characterization of pure compounds of ethanolic extract of Cinnamomum tamala Nees & Eberm leaves. |

| |Screening of the ethanolic extract and isolated compound(s) for their in vitro anthelmintic activity2. |

| | |

| | |

| | |

| |Review of literature: |

| |Anand Murari Saxena et al., (2009)7 studied the antidiabetic potential of the 95% ethanolic extract Cinnamomum tamala Nees leaves was assessed |

| |in single oral dose of 250mg/kg body weight in normal fasted, fed, glucose loaded and Streptozotocin-induced diabetic male albino rats. Diabetes|

| |was confirmed by checking urine glucose by diastex after 48 hours of Streptozotocin injection. Further, effect of crude extract was studied on |

| |muscle glycogen content and histology of pancreatic beta-cells granularity of normal rats to probe in to its mechanism of action. |

| |Varsha Dhulasavant et al., (2011)8 carried out the investigation on the hypolipidemic effect of Cinnamomum tamala Nees leaves extracts in high |

| |cholesterol diet induced male albino rat (wister strain) to cause hyperlipidemia. Aqueous and ethanolic extracts of leaves of Cinnamomum tamala |

| |Nees were administered in doses of 400mg/kg /day p.o. each for 10 days. Simultaneous administration of Cinnamomum tamala Nees leaves extracts |

| |were shown to be significantly (p ................
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