INTRODUCTION - University of Texas at Arlington
Saline. Procedure. Weigh 2 mice (in gm) and number them. Pretreat one mouse with morphine 5mg/Kg sc and the other with saline (0.1ml) sc. Note time of injection. 30 minutes after injection of drug/saline, inject 0.3ml of 0.6% acetic acid ip to each mouse with 26g needle. Place the mouse on the top of a stool for better observation. ................
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