INTRODUCTION - The Lancet

Subsequently, plates were incubated with biotinylated goat anti-human IgE at 1:1000 dilution (Vector, Burlingame, CA, USA) for 1 h and streptavidin-peroxidase (Sigma-Aldrich, St. Quentin Fallavier, France) at 1:1000 dilution for IgE antibody detection. Plates were washed 3 times with PBST between each step. Color was developed using 3,3(5,5(-tetramethylbenzidine substrate solution (Kirkegaard ... ................
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