Materials and Methods - Nature Research

Propidium iodide (Sigma) or DAPI (Invitrogen) was used to distinguish live cells from dead cells during cell analysis and sorting. Stained cells were analyzed on a FACS Canto or LSRII machine using Diva program (BD Bioscience) or purified with a MoFlo Astrios cell sorter (DakoCytomation). Cells were > 98% pure after sorting. ................
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