Introduction to Enzyme Kinetics: Assay of -galactosidase

The lactose fits comfortably into the active site of the enzyme, but its very presence there causes a change in shape of the enzyme. The new conformation puts stress on the bond between the two monosaccharides, the bond breaks, and the products dissociate from the enzyme (Fig 1.). ... Its synthesis is turned on in bacteria when there is not ... ................
................