Urine Collection Urine Jug Protocol for 24-Hour Urine ...

Urine Collection

Urine Jug Protocol for 24-Hour Urine Collections and Timed Collections

Look on the urine list for proper jug; after you have determined the correct jug, see the appropriate section for jug. See the Collection

Manual for tests not on the list below. For inpatients, the laboratory staff will review ordered tests and evaluate the preservative

needed. The jugs that contain a preservative may be labeled with a warning. After assigning the jug, write the patient¡¯s name (first

and last), patient¡¯s identification number, test name, and jug preservative on the jug. Outpatients or the nursing unit should indicate

the start time.

Acceptable Preservatives for Urine Collections

(Analytes that share common preservatives can be assayed using the same specimen)

6N HCl

Boric

Acid

Aldosterone

N

Y

Amino Acid, Quantitative

N

N

Aminolevulinic Acid

Y

N

Analyte

Amylase

Arsenic

Y

N

Beta-2-Microglobulin

Calcium

Y

Cortisol Free

N

Chloride

P

Y

Cystine

Creatinine Clearance

Catecholamines Fractionation

Y

Y

Heavy Metals

Y

N

Histamine

Y

N

Iron

Y

N

Lead

Y

Y

Magnesium

Y

Y

Mercury

Y

N

Metanephrines Fractionation

Y

Y

Myoglobin

Organic Acids

Oxalates

Y

Y

Porphobilinogen (Random Only)

N

N

Porphyrins Fractionation

N

N

Vanillylmandelic Acid (VMA)

Y

Y

Zinc

Y

N

Uric Acid

P=Preferred

Y=Acceptable

N=Not Acceptable

Acetic

Acid

None

Jug

P

N

N

P (freeze)

P

N

Y

Y

P

X - acid washed obtain

from laboratory

Y

P

Y

N*

X - acid washed obtain

from laboratory

P

Y

Y

P

N

Y

P

X - acid washed obtain

from the laboratory

N

P

Y

Y

Y

P

X - acid washed obtain

from laboratory

Y

P

Y

P

X - acid washed obtain

from laboratory

P

N

Y

Y

Y

N*

N

P

N

N

B - sodium carbonate

Y

P

N*

Y

P

X - acid washed obtain

from laboratory

Protect

from Light

Department

Referral

Referral

Referral

Chemistry

Referral

Referral

Chemistry

Referral

Chemistry

Referral

Chemistry

Referral

Referral

Yes

Referral

Referral

Referral

Chemistry

Referral

Referrals

Chemistry

Referrals

Referrals

Yes

Referrals

Yes

Referrals

Chemistry

Referrals

Referrals

*Can add acid within 4 hours of collection.

X - Jugs with no metal cap or glued insert in cap are acceptable.

Check individual test for specimen container and additive requirements. REFRIGERATE all urines if they are stored before delivery

to the laboratory.

Clean-Catch Urine - Female Patients

1. Separate skin folds around urinary opening.

2. Wash urinary opening and its surroundings from front to back with a sterile antiseptic pad.

3. Rinse with clean water on gauze to avoid contamination from soap.

4. Begin urinating into toilet, making sure to keep skin folds apart with fingers on 1 hand.

5. Wait until urine stream is well established before moving container into path of the stream to catch rest of urine.

6. Do not touch cup to genital area.

7. Replace lid and label specimen.

8. Refrigerate specimen immediately.

Clean-Catch Urine - Male Patients

1. Wash end of penis well with a sterile antiseptic pad.

2. Rinse to avoid contamination with soap.

3. Begin urinating into toilet. Wait until urine stream is well established before moving container into path of the stream to catch

rest of urine.

4. Do not touch cup to genital area.

5. Replace lid and label specimen.

6. Refrigerate specimen immediately.

24-Hour Urine Collection

WARNING - Many 24-hour urine collections require an acid solution to preserve specimen. Patients should be cautioned NOT to

urinate directly into container due to possibility of acid burns. Containers are provided by the laboratory. At the time the container

is picked up, the appropriate preservative will be placed in the container, if applicable.

1. Record time collection is begun.

2. Have patient empty bladder at beginning of collection period and DISCARD urine.

3. SAVE all urine for next 24 hours.

4. At end of 24-hour period, empty bladder and SAVE urine.

5. Record time at end of collection.

6. If an aliquot of urine is requested, mix urine well, measure and record total volume and send requested volume of urine.

7. Refrigerate all 24-hour urine specimens during collection, and if storage is required prior to transport to laboratory.

General

? Early-morning urine specimens are preferred for routine urinalysis, but if this is not possible in cases of STATS and pre-Ops,

send specimen to laboratory when requested by physician. SPECIMENS SHOULD NOT BE HELD ON NURSING UNIT

ANY LONGER THAN 30 MINUTES.

? Bladder lavage is completely unsatisfactory if obtaining a catheterized urine specimen.

? For collection of catheterized urine specimens, use only sterile urine containers which will be found on catheter tray and may

also be secured from Supply Processing and Distribution.

? Collect infant urine specimens in pedi-bags or plastic urine containers. Specimens wrung out of cotton balls, diapers, sheets,

etc., are unacceptable.

? Urine specimens for bile and urobilinogen should be taken to laboratory IMMEDIATELY AFTER COLLECTION. Be sure

to let someone in laboratory know what specimens are for, so that they will be given immediate attention.

? Any specimen intended for culture must be collected under strictly sterile conditions and brought to laboratory immediately.

Stool specimens are an exception and should be brought in regular stool containers.

? An out patient that needs a catheterized urine will be sent to Emergency Services triage. Patient will be admitted and an

Emergency Services RN will collect appropriate specimen.

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