Biochemistry Study Guide – Exam 1
Biochemistry Study Guide – Exam 1
This is not a comprehensive list, but simply highlights some of the major topics!
Also, answer all recommended questions at the end of the chapter!
Chapter 1: The chemical basis of life
Domains of life
4 major biomolecules: recognize structures and know properties
3 major types of polymers
Classes of biochemical reactions: understand and be able to recognize reaction type
Nucleophilic substitution (including hydrolysis reactions)
Elimination
Addition
Isomerization
Oxidation-reduction
Overview of metabolism and metabolic regulation
Chapter 2: Living cells
Basic themes of living systems
Water
Membranes
Self-assembly
Molecular machines
Macromolecular crowding
Signal transduction
Prokaryotic cells
Eukaryotic cells
Plasma membrane, ER, Golgi, Mitochondria, Cytoskeleton
Chapter 3: Water: the matrix of life
4 types of weak interactions in water
Ionic bonds, hydrogen bonds, Van der Waals forces (3 types)
Also, hydrophobic interaction (which is not an attractive force)
Acid-base chemistry and ionization
Relationship between [H+] and pH
Acid dissociation constant, pKa
Titration curves for single pKa, multiple pKa’s.
Using the Henderson-Hasslbalch equation
Ocean acidification
Chapter 4: Energy and Metabolism: Thermodynamics
Thermodynamics
1st and 2nd laws
Energy, entropy, enthalpy
Equations for enthalpy change
Free energy and free energy changes
Endergonic and exergonic reactions
Relationship between free energy and the equilibrium constant
Role of ATP (phosphate group transfer potential)
Physical connection of ATP hydrolysis to endergonic reaction
Coupled reactions: exergonic reactions can pull or push endergonic reactions in a metabolic pathway
Activation energy, reaction coordinate diagram, and enzyme function
Chapter 5: Amino acids, peptides and proteins
Amino acid structure
Chirality, enantiomers
Amino acid structure and chemical properties, other info provided in class
Ionization of amino acids at different pH’s, net charge, titration curves
Peptide formation, peptide bond structure
Protein structure: primary, secondary, tertiary and quartenary
Bond rotation, steric hindrance
Alpha helix, beta sheet structure
Protein folding
Protein folding diseases
EXAM 1 Material Ends Here
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