Technical Manual, 20th Edition: Methods and Appendices

Allow 10 to 15 minutes for the clot to form. Use standard centrifugation to separate the clot and serum. Gently agitate the separated serum with small glass beads, at 37 C, for several minutes. ... ABO typing and ABO compatibility testing remain the foundation of pretransfusion testing and an important component of typing before transplantation ... ................
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