Overview of antibody cloning

Samples were analyzed using a 5% to 60% gradient at a flow rate of 300 nL/min over 85 min. Solvent A was 97.8 % water, 2% acetonitrile and 0.2% formic acid; solvent B was 80% acetonitrile, 19.8% water and 0.2% formic acid. Data were acquired in data-dependent mode using the top 15 peaks as previously described [S. 78]. ................
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