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Supplemental Table 1

Supplement Table 1. Real-time quantitative RT-PCR primers used in the present study.

|Gene |Forward |Reverse |

|Nppa |5’-ACCTGCTAGACCACCTGGAG-3’ |5’-CCTTGGCTGTTATCTTCGGTACCGG-3’ |

|Nppb |5’-GAGGTCACTCCTATCCTCTGG-3’ |5’-GCCATTTCCTCCGACTTTTCTC-3’ |

|Myh7 |5’-CCGAGTCCCAGGTCAACAA-3’ |5’-CTTCACGGGCACCCTTGGA-3’ |

|TGF-β1 |5’-AACAACGCCATCTATGAG-3’ |5’-TATTCCGTCTCCTTGGTT-3’ |

|Col1a1 |5’-AGGCTTCAGTGGTTTGGATG-3’ |5’-CACCAACAGCACCATCGTTA-3’ |

|Col3a1 |5’-CCCAACCCAGAGATCCCATT-3’ |5’-GAAGCACAGGAGCAGGTGTAGA-3’ |

|Ctgf |5’-TGACCCCTGCGACCCACA-3’ |5’-TACACCGACCCACCGAAGACACAG-3’ |

|MCIP1 |5’-CGATGATGTCTTCAGCGAAA-3’ |5’-TGGTGTCCTTGTCATATGTTCTG-3’ |

Nppa, atrial natriuretic polypeptide; Nppb, brain natriuretic peptide; Myh7, myosin heavy chain 7; TGF-β1, transforming growth factor-β1; Col1a1, collagen type I; Col3a1, collagen type III; Ctgf, connective tissue growth factor; MCIP1, modulatory calcineurin inhibitory protein 1.

Supplemental Table 2

Supplemental Table 2. Anatomic data for Vehicle and ARO mice under sham or TAC conditions.

|Parameters |Vehicle-Sham |IRN-Sham |Vehicle-TAC |IRN-TAC |

|Number of mice |n=12 |n=10 |n=13 |n=11 |

|Body weight (g) |27.31± 0.51 |27.15±0.68 |26.78±0.82 |27.04± 1.19 |

|Left ventricular + septum weight (mg) |98.60±3.62 |98.45±3.55 |161.34±7.63* |138.25±5.58*# |

|Ventricular weight (mg) |121.44±3.87 |120.31±4.42 |194.36±9.12* |162.13±7.26*# |

|Lung weight (mg) |136.15±3.55 |138.23±4.01 |229.42±21.77* |178.54±19.85* |

|Ratio of LV weight to body weight (mg/g) |3.65±0.08 |3.62±0.08 |6.08±0.31* |5.12±0.25*# |

|Ratio of Ventricular weight to body weight (mg/g) |4.51±0.10 |4.51±0.11 |7.22±0.34* |6.01±0.30*# |

|Ratio of Lung weight to body weight (mg/g) |4.98±0.14 |5.02±0.17 |8.47±0.48* |6.18±0.26*# |

|Tibia length (mm) |17.84±0.06 |17.89±0.11 |17.86±0.10 |17.80±0.15 |

|Ratio of LV weight to Tibia length (mg/mm) |5.51±0.21 |5.37±0.15 |8.96±0.45* |7.60±0.31*# |

|Ratio of V weight to Tibia length (mg/mm) |6.71±0.26 |6.63±0.19 |10.68±0.43* |8.89±0.36*# |

|Ratio of Lung weight to Tibia length (mg/mm) |7.59±0.22 |7.71±0.21 |12.88±1.38* |9.98±0.91* |

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