Real-time (TaqMan®) RT-PCR Assay for the Detection of ...
Make a 1:1000 dilution of reference RNA stock (1 µg/µl) in a 1.5 ml microcentrifuge tube on ice using nuclease-free water to obtain a working concentration of 1 ng/µl. Dilution should be made fresh from stock for each run. Add 1 µl/well of diluted RNA and qs to sample volume with nuclease-free water as indicated on sample plate worksheet. ................
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