Interpretation of a Culture and Sensitivity Report
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Interpretation of a Culture and Sensitivity Report
JESSICA THOMPSON, PHARMD, BCPS (AQ-ID)
Goals
Briefly review the interpretation of culture and sensitivity reports
Example C/S report
Tracheal aspirate: Pseudomonas aeruginosa
Antibiotic
MIC
Interpretation
Aztreonam
8
S
Ceftriaxone
> 32
R
Ceftazidime
4
S
Ciprofloxacin
1
S
Gentamicin
2
S
Meropenem
1
S
Piperacillin/tazobactam
16
S
Interpreting a C/S Report
WHAT IT TELLS YOU
WHAT IT DOES NOT TELL YOU
Identifies bacteria/fungus present (most of the time)
Sensitivity results based on lab data
Human vs lab
Does not identify infection vs colonization vs contamination
Don't treat colonization or contamination
Does not tell you which antibiotic to use
"Susceptibility testing is an in vitro phenomenon and does not necessarily reflect or predict in vivo efficacy. Susceptibility testing is subject to great variability depending on pathogen tested, media used, conditions of incubation, and method of accessing bacterial growth"
BURKE A CUNHA
Example C/S report
Tracheal aspirate: Pseudomonas aeruginosa
Antibiotic
MIC
Interpretation
Aztreonam
8
S
Ceftriaxone
> 32
R
Ceftazidime
4
S
Ciprofloxacin
1
S
Gentamicin
2
S
Meropenem
1
S
Piperacillin/tazobactam
16
S
definitions
MIC
? Minimum concentration of an antibiotic needed to inhibit
visible growth of a single isolate of an organism
? Important for definitive treatment of an individual patient
Breakpoint ? Discriminatory concentrations used in the interpretation of
results of susceptibility testing to define isolates as susceptible,
intermediate, or resistant (determined by various organizations
- FDA, CLSI, EUCAST)
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