Aseptic Technique - Jose de Ondarza
Chapter 2 Data Sheet: Aseptic Technique
1. Observe your TSB tubes before practicing aseptic technique and after incubation. Describe – are the broths clear or cloudy?
a. Before:
b. After:
2. Practice pipetting 8.0 ml of distilled water into a plastic dish (on the scale), once with the blow-out pipette and once with the to-deliver pipette. Write down how much the water weighed.
a. Blow-out pipette: __________ g
b. To-deliver pipette: __________g
3. Practice pipetting with the micropipettors. Record the weight of the water you pipetted here:
a. 800 μl (0.8 ml) using the P1000: _________ g
b. 80 μl (0.08 ml) using the P200: _________ g
Review Questions
1. List 4 things you can do to minimize the risk of contamination during aseptic transfer of cultures.
2. How can you tell whether your TSB broth is contaminated or not?
3. What is the difference between a blow-out pipette and a to-deliver pipette?
4. Which of these is more accurate? Why?
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