Lecture 11 - Biosynthesis of Amino Acids
Lecture 11 - Biosynthesis of Amino Acids
Chem 454: Regulatory Mechanisms in Biochemistry University of Wisconsin-Eau Claire
Introduction
Biosynthetic pathways for amino acids, nucleotides and lipids are very old Biosynthetic (anabolic) pathways share common intermediates with the degradative (catabolic) pathways. The amino acids are the building blocks for proteins and other nitrogen-containing compounds
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Introduction
Nitrogen Fixation
Reducing atmospheric N2 to NH3
Amino acid biosynthesis pathways Regulation of amino acid biosynthesis. Amino acids as precursors to other biological molecules.
e.g., Nucleotides and porphoryns
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Introduction
Nitrogen fixation is carried out by a few select anaerobic micororganisms The carbon backbones for amino acids come from glycolysis, the citric acid cycle and the pentose phosphate pathway. The L?stereochemistry is enforced by transamination of ?keto acids
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1. Nitrogen Fixation
Microorganisms use ATP and ferredoxin to reduce atmospheric nitrogen to ammonia.
60% of nitrogen fixation is done by these microorganisms
15% of nitrogen fixation is done by lighting and UV radiation.
25% by industrial processes
Fritz Habers (500?C, 300 atm)
N2 + 3 H2
2 N2
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