Co-Cultivation Medium
Co-Cultivation Medium (CCM)
Prepared by:_____________
Date:_____________
|Ingredients |Volume |Actual amt added |
| |1 L |2 L |3 L |4 L | |
|10x B5 Major (1X final) |10ml |20ml |30ml |40 ml | |
|4°C fridge | | | | | |
|100x B5 Minor (1X final) |1.0ml |2.0ml |3.0ml |4.0ml | |
|4°C fridge | | | | | |
|100x FeSO4/NaEDTA |1.0ml |2.0ml |3.0ml |4.0ml | |
|(MSIII Iron) (1X final)) | | | | | |
|4°C fridge | | | | | |
|MES (20mM final) |3.9g |7.8g |11.7g |15.6g | |
|Cabinet (Sigma cat# M8250 (1kg); M2933 (100g)) | | | | | |
|Sucrose (3% final) |30g |60g |90g |120g | |
|Cabinet | | | | | |
| |
|pH 5.4 pH= |
|Agar (0.5% final) |5g |10g |15g |20g | |
|Cabinet (Sigma cat# A-1296) | | | | | |
|Final volume |980ml |1960ml |2940ml |3920ml | |
Wear protective gloves
Use 100x15ml Petri dishes. (thin dishes)
Co-Cultivation add-ins
|Ingredients |Amount of media |Actual |
| |1L |2L | |
|BAP (BenzylAminoPurine Sol.) (1.67mg/L final) |1.67ml |3.34mll | |
|4°C fridge (PhytoTechnology labs cat# B130-100mL) | | | |
|GA3 (Gibberellic acid) (0.25mg/L final) |0.25ml |0.50ml | |
|4°C fridge (Sigma cat# G-7645) | | | |
|100x B5 vitamins (1/10 strength final) |10ml |20ml | |
|4°C fridge | | | |
|DTT (1mM final) |0.154g |7.7mg | |
|4°C fridge | | | |
|Cysteine (8.3mM final) |1g |2g | |
|Cabinet | | | |
|Acetosyringone (AS) (200um final) |2 mL |4mL | |
|4°C fridge 100mM solution | | | |
................
................
In order to avoid copyright disputes, this page is only a partial summary.
To fulfill the demand for quickly locating and searching documents.
It is intelligent file search solution for home and business.
Related searches
- medium hairstyles for women over 50
- medium shaggy hairstyles for women over 50
- medium hairstyles for women over 60
- grade 6 english medium papers
- biology model papers in sinhala medium 2019
- medium sized business revenue
- co signer vs co borrower
- grade 6 english medium health
- sinhala medium grade 06
- sinhala medium grade 4
- what is a medium correlation coefficient
- psychic medium symbols