Chromatography - Sheryl Hoffmann
Activity 1 – Practice
1. Read “Basic Method for Paper Chromatography” & “Spotting Methods”
2. Practice spotting food colours onto on a filter paper until you are able to produce a small, dark spot. Try each of the following & see which you find works best:
• regular toothpick
• catering (pointy) toothpick
• capillary tube
• pasteur pipette
3. Have a look at a piece of TLC sheet. Scrape a little of the silica gel off the backing sheet with your thumbnail.
4. Try spotting food colours on a TLC sheet
Activity 2 – Comparison of 1M NaCl & Water in Separating Food Colours
1. Prepare a piece of chromatography paper for use in a 250ml beaker.
2. Spot each of three colours & a mixture of all colours onto a piece of paper.
3. Run the chromatography in either 1M Sodium Chloride or water.
4. Compare your results with someone who used the opposite solvent.
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Activity 3 – Chromatography Using Sticks of Chalk
Chalk is a self supporting column that can be used for column chromatography.
Method
Draw a line around the chalk 2 cm from the larger end either with a mixture of food colours or with a coloured texta. Stand the wider end in a Petri dish containing ................
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