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Fractions containing Cas9 were pooled and concentrated to 10-20 mg/ml and aliquots flash frozen in liquid nitrogen. For working stocks dCas9 was diluted to 1.6 μg/μl in 300 mM NaCl, 10 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol and stored at -20˚C. Single Molecule Experiments ................
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