Technical Manual, 20th Edition: Methods and Appendices

0.1 M glycine-HCl buffer (pH 1.5) prepared by diluting 0.75 g of glycine to 100 mL with isotonic (unbuffered) saline. Adjust the pH to 1.5 using concentrated HCl. 1.0 M TRIS-NaCl prepared by dissolving 12.1 g of Tris(hydroxymethyl)aminomethane (TRIS) and 5.25 g of sodium chloride (NaCl) to 100 mL with distilled or deionized water. ................
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