Recipes and Reagents
Sucrose Lysis Buffer for miniprep (50ml) [Stock] amount [final] sucrose 4g 8% . Triton x-100 0.25ml 0.5% (very viscous, weigh out) 0.5M EDTA 5.0ml 50mM. 1M Tris pH8.0 0.5ml 10mM. H20 enough to make 50ml TAE 50x Gel Buffer. For 1 liter . 242.0 g Tris Base. 57.1 ml … ................
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