Cryoprotectant

0.1M Phosoahte buffer pH 7.2 500ml 250ml 100ml 50ml NaH2PO4H2O 1.59 g 0.80 g 0.32 g 0.16 g Na2HPO4 5.47 g 2.74 g 1.09 g 0.55 g NaCl 9.00 g 4.50 g 1.80 g 0.90 g Sucrose (30%) 300.00 g 150.00 g 60.00 g 30.00 g Polyvinylpyrolidone (1%) 10.00 g 5.00 g 2.00 g 1.00 g ethylene glycol (30%) 300ml 150ml 60ml 30ml Adjust ddH20 to: 1000ml 500ml 200ml 100ml ................
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