The Science and Application of Hematoxylin and Eosin Staining
The Science and Application of Hematoxylin and Eosin Staining
Skip Brown, M.Div, HT (ASCP)
Robert H. Lurie Comprehensive Cancer Center Northwestern University
hsb467@e.northwestern.edu
Living up to Life
Stains and Staining Systems
Routine Staining
(Hematoxylin & Eosin)
Nuclear detail/definition - Hematoxylin Contrasting counterstain - Eosin
The Hematoxylin and Eosin Stain
? Long history of use
? Mayer 1904
? Primary diagnostic technique in the histopathology laboratory.
? Primary technique for the evaluation of morphology
? Widely used
? 2.5 to 3 million slides per day
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