Rodent Survival Blood Sampling Information



Guidelines for SEQ CHAPTER \h \r 1Rodent Survival Blood CollectionReviewed and Approved by FAU IACUCEffective Date: 04 December 2015 Last Reviewed/Revised: N/AMouse blood volume is normally 72 ml/kg body weight (0.072ml/g body weight) - assuming the animal is mature, healthy, and on an adequate plane of nutrition. A drop of blood can be 15-20?l (small) or 25-30?l (large) but it is not possible to accurately quantify the amount.Rat blood volume is normally 64 ml/kg body weight (0.064ml/g body weight) - assuming the animal is mature, healthy, and on an adequate plane of nutrition. The NIH's “Guidelines for Survival Bleeding of Mice and Rats” contain the following:“Both the quantity and frequency of blood sampling are dependent on the circulating blood volume of the animal. The approximate blood volume of a mouse is 72 ml/kg, ± 8 ml ... (e.g., 1.5 ml for a 20 gram mouse...) In general, no more than 10% of the animal’s blood volume should be removed at one sampling....” Approximate Blood Sampling Volumes and Recovery PeriodsSingle SamplingMultiple Sampling% Circulatory Blood Volume RemovedApproximate Recovery Period% Circulatory Blood Volume Removed in 24 hoursApproximate Recovery Period7.5%1 Week7.5%1 Week10%2 Weeks10-15%2 Weeks 15%4 Weeks20%4 WeeksPer information extracted from McGuill, M.W. and Rowan, A.N., "Biological Effects of Blood Loss: Implications for Sampling Volumes and Techniques," ILAR News, Vol. 31(4), Fall 1989, pp 5-20:Recommendations for a Single Blood Sample:Limit a single sampling to 15% of the total blood volume and allow a 30-day recovery period. Up to 20% of the blood volume may be feasible in an animal in prime health that is given replacement fluids (4 ml/kg IV isotonic saline). In general, larger animals have a lower blood volume than smaller animals. If the total blood volume of an animal is unknown, a rough "rule of thumb" is that 6% of the body weight is blood volume. Mouse blood volume is normally 72 ml/kg body weight - assuming the animal is mature, healthy, and on an adequate plane of nutrition. Thus, a safe maximum blood sample for a 20 gram mouse would be 0.15 ml.Rat blood volume is normally 64 ml/kg body weight, assuming the animal is mature, healthy, and on an adequate plane of nutrition. Thus, a safe maximum blood sample for a 300 gram rat would be 1.15 ml.Blood loss of 30% of blood volume, or greater, is life threatening. The common 10 percent-10 percent rule (this estimates a safe volume as 10% of the total blood volume, and that the total blood volume is approximately 10% of the body weight) will result in an excessive blood loss.MOUSE Blood Sampling VolumesMaximum Allowable Blood Sample Size (ml)Body WeightIn GramsTotal Blood Volume (ml) (TBV = 7% BW)Weekly (7.5% TBV)Every Two Weeks(10% TBV)Every Four Weeks(15% TBV)151.050.080.110.16161.120.080.110.17171.190.090.120.18181.260.090.130.19191.330.100.130.20201.400.110.140.21211.470.110.150.22221.540.120.150.23231.610.120.160.24241.680.130.170.25251.750.130.180.26261.820.140.180.27271.890.140.190.28281.960.150.200.29292.030.150.200.30302.100.160.210.32RAT Blood Sampling VolumesMaximum Allowable Blood Sample Size (ml)Body WeightIn GramsTotal Blood Volume (ml) (TBV = 7% BW)Weekly (7.5% TBV)Every Two Weeks(10% TBV)Every Four Weeks(15% TBV)251.630.120.160.24352.280.170.230.34452.930.220.290.44553.580.270.360.54654.230.320.420.63754.880.370.490.73 855.530.410.550.831006.500.490.650.981258.130.610.811.221509.750.730.981.4617511.380.851.141.7120013.000.981.301.9525016.251.221.632.4430019.501.461.952.9335022.751.712.283.4140026.001.952.603.9045029.252.192.934.3950032.502.443.254.88 ................
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