Psoralen (AMT) Crosslinking - California Institute of ...

Psoralen (AMT) Crosslinking. Grow adherent cells on 15-cm plates. Prepare plates for both crosslinked (+AMT) and mock-crosslinked (-AMT control) conditions. Prepare 0.5 mg/mL AMT solution in PBS: Resuspend AMT in H. 2 O at 1 mg/mL and then add an equal volume of 2× PBS. Chill solution in the dark on ice. Remove media from cells. Rinse cells in plate with 10 mL room temperature PBS. Discard ... ................
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