Technical Manual, 20th Edition: Methods and Appendices

0.1 M stock 2-ME: 0.7 mL of a 14 M stock solution of 2-ME diluted in 100 mL of PBS at pH 7.3; 2-ME should be stored in a dark glass container at 4 C. PBS at pH 7.3. Antigen-positive control red cells for antigen typing to be treated in parallel. ................
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