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studier@bnl.gov) growth media for T7 E. coli strains (8/20/02) General Information. The following media and protocols have been developed for use with DE3 lysogens such as BL21(DE3) and B834(DE3), which supply T7 RNA polymerase upon induction of the lacUV5 promoter, in combination with pET expression vectors in which expression of the target gene is controlled from the T7lac promoter … ................
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