Epitope discovery from natural peptide libraries: a new ...
E. coli strain BL21(DE3) cells (Novagen) carrying the fusion plasmids were inoculated into 300 ml (IPIII-His fusions) or 900 ml (MBP fusions) LB medium (Sambrook et al., 1989) containing kanamycin at 50 µg/ml (the plasmid vector confers resistance to this drug). ................
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