SiRNA Cassette protocol - GenScript
Commonly used competent E. coli cells, such as DH5α, BL21(DE3) competent cells, can be used for this purpose. Thaw E. coli DH5α (or BL21(DE3)) competent cells on ice. Pipet 50 µl of DH5α competent cells into 1.5 ml microcentrifuge tube containing the annealing mixture. Gently mix well and incubate on ice for 30 minutes. ................
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