The Inoue Method for Preparation and Transformation of ...
Shock cells with Bio-Rad electroporator set on EC1 (for E. coli, 1 mm cuvette, which is the size we have in our lab). Immediately after shocking a cuvette, go to step 5 (i.e., don’t shock all before going to step 5). If the electroporator shows “arc” after shocking your cells, you … ................
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