Supplementary Methods - Home | Clinical Cancer Research - Hypoxia chamber for tissue culture

chemiluminescence and detection with the Bio‑Rad Molecular Imager Gel Doc. For the hypoxia experiments: single cell suspensions of M7, HF2303 and U251 cells were plated onto T25 tissue culture flasks in 7ml NSC media at a density of 5x104 cells/ml for M7 and 2x105 cells/ml for U251 and HF2303. ................
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