CURRICULUM VITAE



CURRICULUM VITAE

Name: 026078311 Lecturer ZOR TSAFFRIR

Faculty / Department: Life Sciences / Biochemistry

03-640-7192

Home address: KFAR MENACHEM 22, KFAR MENACHEM 79875, Israel

08-858-4684

Date and place of birth: 11/8/66, USA

Date of arrival to Israel: 1967

Military Service: 1985-1988

Marital status and no. of children: married + 2

A. Education

|Period of study |University Name |Subject |Degree |Award Date |

|1998-1991 |The Hebrew University, Jerusalem |Chemistry |B.Sc. |1992 |

| | | |(summa cum laude, 97) | |

|1991-1997 |The Hebrew University, Jerusalem |Biochemistry |Ph.D. |1998 |

| | | |(summa cum laude) | |

B.Sc. Thesis title ("Amirim" outstanding students program) - "photoregulation of enzyme activity in photochromic polymer", grade - 100.

Supervisor – Itamar Wilner

Ph.D. dissertation title - "Development of tools for identification and characterization of guanine nucleotide binding proteins activity".

Supervisor – Zvi Selinger

C. Academic and Professional Experience

1) 1990: Hebrew University, Jerusalem, Israel

Crystallography of a cobalt complex and kinetics of lignad substitution (article #1)

Dept. of Inorganic chemistry

Project student

2) 1990: Hebrew University, Jerusalem, Israel

Photoregulation of enzyme activity in photochromic polymer (articles #2-3)

Dept. of Organic chemistry

"Amirim" outstanding students program – research project

3) 1991: Hebrew University, Jerusalem, Israel

Development of a photo-affinity label for G proteins (article #4)

Dept. of Biological chemistry

Project student

4) October 1991- November 1997: Hebrew University, Jerusalem, Israel

Development of tools for the study of G proteins activity (articles #4-8 & review #1)

Dept. of Biological chemistry

Ph.D. student

5) January 1998 – May 2003: The Scripps Research Institute, La-Jolla, CA, USA

Structural (protein NMR) and biophysical studies of a transcriptional activator: co-activator complex (articles #9-13)

Dept. of Molecular Biology

Post-doctoral fellow

6) June 2003- July 2004: Genomics Institute of the Novartis Foundation, La-Jolla, CA, USA

Regulation of in-vivo TNFα production (continued collaboration leading to articles #14-15)

Dept. of Chemistry

Institute fellow

7) June 2003- July 2004: The Scripps Research Institute, La-Jolla, CA, USA

Regulation of in-vivo TNFα production (continued collaboration leading to articles #14-15)

Dept. of Immunology

Guest scientist

8) August - September 2004: Tel-Aviv University, Tel-Aviv, Israel

laboratory set-up

Dept. of Biochemistry

Visiting Lecturer

9) October 2004 – October 2009 (activity since 4/2005): Tel-Aviv University, Tel-Aviv, Israel

Regulation of cytokine production in macrophages (articles #14-18)

Dept. of Biochemistry

Lecturer

10) November 2009 - now: Tel-Aviv University, Tel-Aviv, Israel

Regulation of cytokine production in macrophages (articles #19-20 +2 submitted +2 in preparation)

Dept. of Biochemistry

Senior Lecturer

D. Active participation in scientific meetings

1993: (poster) Jacques Monod conference on "molecular and cellular biology of guanine nucleotide-binding regulatory proteins", Aussois , France

1994: (poster) Third annual meeting of the Israel society for neuroscience, Eilat, Israel

1995: (poster) Fourth annual meeting of the Israel society for neuroscience, Eilat, Israel

1996: (poster) Jacques Monod conference on "molecular and cellular biology of ras-like and heterotrimeric G proteins", Aussois, France

1996: (poster) Gordon conference on "Biocatalysis", NH, USA

2000: (lecture) EMBO fellows meeting, Heidelberg, Germany

2001: (lecture) International society of magnetic resonance (ISMAR), Rhodes, Greece

2005: (poster) Keystone symposium on "cytokines, Disease and Therapeutic Intervention", Santa-Fe, NM

2006: (lecture) Annual meeting of the Israeli Club for Magnetic Resonance, Tel-Aviv, Israel

2008: (posters) 5'th congress of the federation of Israel Societies for Experimental Biology, Eilat, Israel

2008: (poster) 15'th Inflammation Research Association (IRA) conference, Chantilly, VA, USA

2008: (lecture) 7'th Sphingolipid club conference, Leiden, Netherlands

2009: (posters) Israel Immunological Society 37'th annual meeting

2009: (lecture) 5'th Charleston ceramide conference, Charleston, SC, USA

2010: (lecture) 8'th Sphingolipid club conference, Glasgow, UK

2010: (lecture) 51'th International Conference on the Bioscience of Lipids, Bilbao, Spain

E. Academic and professional awards

E.1 Internal grants (at TAU)

|Year |Foundation |Title |Sum |Status |

|2006 |TAU Research Authority |High score in the ISF grant |19,000 NIS (~$4,400) |PI |

|2008 |TAU Research Authority |High score in the BSF grant |27,000 NIS (~$7,500) |PI |

|2009 |TAU Research Authority |High score in the ISF grant |21,000 NIS (~$5,600) |PI |

E.1.2 External grants

|Year |Foundation |Title |Sum |Status |

|2005 |European Commission FP6 – Marie Curie |Mechanism of anti-inflammatory activity of a |80,000 euro |PI |

| |IRG |phospholipid-like drug |(~$100,000) | |

|2006 |Teva Pharm. Ind. Ltd. – |A phospholipid-dependent anti-inflammatory signal |50,000 NIS |PI |

| |Young investigators grant for research |transduction pathway in macrophages |(~$12,000) | |

| |of auto-immune diseases | | | |

|2007 |Public committee for allocation of |Mechanism of TNFα suppression and anti-inflammatory |200,000 NIS |PI |

| |Estate funds at Israel's ministry of |activity of a phospholipid-like drug |(~$48,000) | |

| |justice | | | |

|2007 |Israel Science Foundation (ISF) |A phospholipid-dependent anti-inflammatory signal |326,000 NIS |PI |

| | |transduction pathway in macrophages |(~$82,000) | |

|2007 |Israel Science Foundation (ISF) |equipment for a new lab |$65,000 |PI |

|2007 |Wolfson Foundation |equipment for a new lab |154,878 pound (~$310,000) |PI |

|2010 |Health ministry of Israel |Identification and characterization of |300,000 NIS |PI |

| | |inflammation-linked receptors for C1P and for its |(~$80,000) | |

| | |analog PCERA-1 | | |

E.2 Fellowships (competitive)

1992-3: Excellence fellowship for M.Sc. students

1997: Rothschild post-doctoral fellowship

1997-9: EMBO post-doctoral fellowship

E.3 Scholarships

1989-1991: Scholarship of the "Amirim" outstanding B.Sc. students program

1996: Katzir travel award for Ph.D. students

2001: ISMAR scholarship for conference lecture

E.4 Prizes

1989: a) Dean's prize for BSc. students in the science faculty

b) Excellence prize - "De-Shalit" chemistry summer school (Weizmann Institute)

1990: Dean's list of the science faculty

1991: Petai prize for five top B.Sc. graduates in the science faculty

1992: a) B.Sc. conferred with "summa cum laude"

b) Rector's prize for M.Sc. students in the science faculty

1994: Landow prize for Ph.D. students

1997: Best poster in the life sciences institute posters contest

1998: Ph.D. conferred with "summa cum laude"

2001: Best poster in the Scripps research institute society of fellows posters contest

2009: Best poster in the Israel Immunological Society meeting (presented by my student)

F. Membership in professional societies

2005-present: Israel Society for Biochemistry & Molecular Biology (ISBMB, Israel)

2008-present: The sphingolipid club (International)

G. Doctoral students supervised (all at TAU)

1) October 2004-August 2010: Dorit Avni

Subject: Regulation of cytokine production in LPS-activated macrophages by PCERA-1

Thesis title: Regulation of TNFα and IL-10 production in LPS-activated macrophages by the cAMP and NFκB p50 pathways

2) October 2004-December 2009: Meir Goldsmith

Subject: Regulation of cytokine and PGE2 production in macrophages by PCERA-1

Thesis title: Identification and characterization of TNFα suppression mechanism by the phospholipid-like molecule PCREA-1

3) October 2006-present (Direct Ph.D. program): Orna Ernst

Subjects: 1) Regulation of cytokine production in macrophages by PCERA-1

2) Regulation of cytokine production in macrophages by Hsp10

Thesis title: Mechanisms of regulation of LPS-induced IL-10 and TNFα expression by PCREA-1 via the p38 and cAMP pathways.

4) October 2007-present: Yifat Glucksam

Subjects: 1) Regulation of IL-10 transcription in macrophages by PCERA-1

2) Regulation of macrophages activity by CD44 and its ligand Hyaluronan

3) PCERA-1 drug delivery by CD44-targeted bioadhesive liposomes

Thesis title: Modulation of macrophages inflammatory responses by the phospho ceramide analog PCERA-1, and by CD44-targeted bioadhesive liposomes.

M.Sc. students supervised (all at TAU)

1) October 2004- October 2006: Elad Bar-David

Subject: Regulation of cytokine production by a PCERA-1-derived metabolite

Thesis title: Purification and identification of the active metabolite of an anti-inflammatory phospholipid-like molecule

2) October 2005- October 2007: Maya Levi

Subject: Regulation of cytokine production by sphingolipids

Thesis title: Comparison of the putative anti-inflammatory activities of PCERA-1 and C8-C1P

3) October 2006- October 2008: Amir Pri-Or

Subject: Regulation of cytokine production by Hsp10

Thesis title: Characterization of the involvement of the mitochondrial co-chaperonin Hsp10, w.t. and mutant L33A, in macrophages function and in interaction with the chaperonin Hsp60

4) October 2007- October 2009: Amir Philosoph

Subject: Regulation of cytokine production by PCERA-1

Thesis title: From the PCERA-1 receptor via adenylyl cyclase to cytokines modulation

5) October 2008- September 2010: Ala Daka

Subject: Regulation of PGE2 production in macrophages

Thesis title: Inhibition of PGE2 production by a ceramide analog

6) October 2009- present: Shahar Cohen

Subject: Regulation of cytokine production by PCERA-1

Thesis title: to be decided

6) October 2009- present: Sharon Avraham

Subject: Melanocortin1 receptor variability – a link with melanoma risk

Thesis title: to be decided

SCIENTIFIC PUBLICATIONS

B.1. ORIGINAL ARTICLES

Articles published

1) B. Ballet, A. Bino, S. Cohen, H. Rubin, T. Zor

A linear mixed oxidation state trinuclear cobalt complex with six bridging sulfito ligands

Inorganica Chimica Acta, 188, pp. 91-93, 1991

Impact factor – 2.0 Citations – 8

2) Itamar Willner, Shai Rubin and T. Zor

Photoregulation of α-chymotrypsin by its immobilization in a photochromic azobenzene copolymer.

Journal of the American Chemical Society, 113, pp. 4013-4014, 1991

Impact factor – 8.1 Citations – 40

3) I. Willner, S. Rubin, R. Shatzmiller, T. Zor

Reversible light-stimulated activation and de-activation of α-chymotrypsin by its immobilization in photoisomerizable copolymers.

Journal of the American Chemical Society, 115, pp. 8690-8694, 1993

Impact factor – 8.1 Citations – 52

4) T. Zor, I. Halifa, S. Kleinhaus, M. Chorev, Z. Selinger

m-Acetyl Anilido GTP, a novel photoaffinity label for GTP-binding proteins: synthesis and application.

Biochemical Journal, 306, 1, pp. 253-258, 1995

Impact factor – 4.4 Citations – 10

5) T. Zor, Z, Selinger

Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies.

Analytical Biochemistry, 236, pp. 302-308, 1996

Impact factor – 3.1 Citations – 295

6) T. Zor, M. Bar-Yaacov, S. Elgavish, B. Shaanan, Z. Selinger

Rescue of a mutant G-protein by substrate-assisted catalysis.

European Journal of Biochemistry, 249, pp. 330-336, 1997

Impact factor – 3.1 Citations – 22

7) T. Zor, R. Andorn, I. Sofer, M. Chorev, Z. Selinger

GTP analogue hydrolysis by the Gs protein: implication for the role of the catalytic glutamine in the GTPase reaction.

FEBS Letters, 433, pp. 326-330, 1998

Impact factor – 3.3 Citations – 15

8) M.R. Ahmadian*, T. Zor *, D. Vogt, W. Kabsch, Z. Selinger, A. Wittinghofer, K. Scheffzek

GTPase stimulation of oncogenic Ras mutants.

Proceedings of the national Academy of Sciences USA, 96, pp. 7065-7070, 1999

Impact factor – 9.4 Citations – 57

* The first two authors contributed equally to this work.

9) D. Parker, M. Rivera, T. Zor, A. Henrion-Caude, I. Radhakrishnan, A. Kumar, L.H. Shapiro, P.E. Wright, M.R. Montminy, P.K. Brindle

Role of secondary structure in discrimination between constitutive and inducible activators.

Molecular and Cellular Biology, 19, pp. 5601-5607, 1999

Impact factor – 5.9 Citations – 71

10) T. Zor, B.M. Mayr, H.J. Dyson, M.R. Montminy, P.E. Wright

Roles of phosphorylation and helix propensity in the binding of the KIX domain of CBP by constitutive (c-Myb) and inducible (CREB) activators.

Journal of Biological Chemistry, 277, pp. 42241-42248, 2002

Impact factor – 5.5 Citations – 43

11) N.K. Goto*, T. Zor*, M. Martinez-Yamout, H.J. Dyson, P.E. Wright

Cooperativity in transcription factor binding to the coactivator CBP: the MLL activation domain binds to an allosteric site on the KIX domain.

Journal of Biological Chemistry, 277, pp. 43168-43174, 2002

Impact factor – 5.5 Citations – 42

* The first two authors contributed equally to this work.

12) T. Zor, R.N. De Guzman, H.J. Dyson, P.E. Wright

Solution structure of the KIX domain of CBP bound to the transactivation domain of c-Myb.

Journal of Molecular Biology, 337, pp. 521-534, 2004

Impact factor – 4.2 Citations – 41

13) J.L. Best, C.A. Amezcua, B. Mayr, L. Flechner, C.M. Murawsky, B. Emerson, T. Zor , K.H. Gardner, M.R. Montminy

Identification of Small Molecule Antagonists that Inhibit an Activator: Coactivator Interaction.

Proceedings of the national Academy of Sciences USA, 101, pp. 17622–17627, 2004

Impact factor – 9.4 Citations – 35

14) M. Goldsmith, D. Avni, G. Levy-Rimler, R. Mashiach, O. Ernst, M. Levi, B. Webb, M.M. Meijler, N.S. Gray, H. Rosen, T. Zor

A ceramide-1-phosphate analogue, PCERA-1, simultaneously suppresses tumour necrosis factor-α and induces interleukin-10 production in activated macrophages.

Immunology, 127, pp. 103-115, 2009 (E-pub 2008, Sep 11)

Impact factor – 3.4 Citations – 3

15) D. Avni, M. Goldsmith, O. Ernst, R. Mashiach, T. Tuntland, M.M. Meijler, N.S. Gray, H. Rosen, T. Zor

Modulation of TNFα, IL-10 and IL-12p40 levels by a ceramide-1-phosphate analog, PCERA-1, in-vivo and ex-vivo in primary macrophages.

Immunology Letters, 123, pp. 1-8, 2009

Impact factor – 2.9 Citations – 4

16) M. Goldsmith, D. Avni, O. Ernst, Y. Glucksam, G. Levy-Rimler, M.M. Meijler, T. Zor

Synergistic IL-10 induction by LPS and the ceramide-1-phosphate analog PCERA-1 is mediated by the cAMP and p38 MAP kinase pathways.

Molecular Immunology, 46, pp. 1979-1987, 2009

Impact factor – 3.6 Citations – 3

17) M. Levi, M.M. Meijler, A. Gómez-Muñoz, T. Zor

Distinct receptor-mediated activities in macrophages for natural ceramide-1-phosphate (C1P) and for phospho-ceramide analogue-1 (PCERA-1).

Molecular and Cellular Endocrinology, 314, pp. 248-255, 2010

Impact factor – 3.6 Citations – 2

18) D. Avni, A. Philosoph, M.M. Meijler, T. Zor

The ceramide-1-phosphate analog PCERA-1 modulates TNFα and IL-10 production in macrophages via the cAMP-PKA-CREB pathway in a GTP-dependent manner.

Immunology, 129, pp. 375-385, 2010

Impact factor – 3.4 Citations – 1

19) D. Avni, O. Ernst, A. Philosoph, T. Zor

Role of CREB in modulation of TNFα and IL-10 expression in LPS-stimulated RAW264.7 macrophages.

Molecular Immunology, 47, pp. 1396-1403, 2010

Impact factor – 3.6 Citations – not available yet

20) O. Ernst, T. Zor

Linearization of the Bradford protein assay

Journal of Visualized Experiments, 38, 2010



doi: 10.3791/1918

Impact factor – not available yet Citations – not available yet

21) M. Goldsmith, A, Daka, N.F. Lamour, R. Mashiach, Y. Glucksam, M.M. Meijler, C.E. Chalfant, T. Zor

A ceramide analog inhibits cPLA2 activity and consequent PGE2 formation in LPS-stimulated macrophages.

Immunology Letters, 135, pp. 136-143, 2011–01–23

Impact factor – 2.9 Citations – not available yet

Accepted for publication

Submitted for publication

1) Y. Glucksam, T. Zor, R. Margalit

Targeting regular and hyaluronan-modified liposomes to macrophages and their impact on TNF-α production.

BBA-Biomembranes, submitted

In preparation

1) D. Avni, Y. Glucksam, T. Zor

LY294002 and LY303511 inhibit LPS-induced IL-10 expression in macrophages via NFkB1 (p50).

Expected submission date: November 2010

2) Y. Glucksam, M.M. Meijler, T. Zor

A pseudomonas aeruginosa quorum-sensing signal molecule up-regulates production of the anti-inflammatory cytokine IL-10 in stimulated macrophages.

Expected submission date: December 2010

B.3. REVIEW ARTICLES

1) M. Kosloff, T. Zor, Z. Selinger

Substrate Assisted Catalysis: implications for biotechnology and drug design.

Drug Development Research, 50, pp. 250-257, 2000

Impact factor – 1.4

D.3. ABSTRACTS AND PROCEEDINGS

1) R. Andorn-Broza, I. Sofer, T. Zor, M. Chorev, Z. Selinger

GTP analog hydrolysis by the G(s) protein: Implications for the role of catalytic glutamine in the GTPase reaction.

Neuroscience Letters, Suppl. 51, S2, 1998

2) D. Avni, M. Goldsmith, T. Zor

Synergistic induction of IL-10 by a TLR agonist and a phospho-ceramide analog is mediated by CAMP.

Inflammation Research, 57, S111-S112, 2008

3) M. Goldsmith, D. Avni, M. Levi, O. Ernst, M.M. Meijler, N.S. Gray, H. Rosen, T. Zor

A ceramide-1-phosphate analog, PCERA-1, suppresses TNF alpha and induces IL-10 production in activated macrophages.

Naunyn-Schmiedebergs archives of pharmacology, 380, 4, 364, 2009    

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