Transcription in Prokaryotes & Eukaryotes
Transcription in Prokaryotes & Eukaryotes
Gene Expression
? There are 4 major events that occur during the process of gene expression
? Transcription ? RNA processing ? Translation ? Protein processing
A Gene is a Transcription Unit
Promoter
Regulatory sequence
Terminator
DNA
Transcription
mRNA 5
Start codon
Ribosomalbinding site
Many codons
3
Stop codon
Prokaryotic Gene Structure
+1
DNA Cis-Regulatory Elements
ShineDelgarno
box ATG
Stop Codon TAA, TAG,
TGA
Coding Sequence = ORF 1
ShineDelgarno
box ATG
Stop Codon
Coding Sequence = ORF 2
USE/Promoter /Operator
5' UTR = Leader Protein A sequence
Cistron 1
Spacer = 5'UTR of 2nd cistron
Protein B
Cistron 2
Regulatory Sequences Structural or Coding Sequences
Termination sequence
Regulatory and Coding Sequence Unit = Operon
Polycistronic structure
Prokaryotic Gene Structure
DNA
+1
Cis-Regulatory Elements
Shine-Delgarno box ATG
Shine-Delgarno
Stop Codon box
TAA, TAG, TGA
ATG
Stop Codon TAA, TAG, TGA
Coding Sequence= ORF
Coding Sequence= ORF
USE/Promoter /Operator
5' UTR = Leader sequence
Protein A
Cistron 1
Spacer = 5'UTR of 2nd cistron
Protein B Cistron 2
Terminator sequence
5' UTR = Leader sequence
Spacer
Polycistronic
mRNA
ORF Protein A
+1
AUG
Shine-Delgarno
box
Stop Codon
AUG Shine-Delgarno
UAA, UAG, UGA
box
ORF Protein B
Stop Codon UAA, UAG, UGA
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