Prokaryotic Transcription - Jiwaji University
SOS in Biochemistry, Jiwaji University, Gwalior M.Sc. II Semester (2019-20) Paper BCH 205: Fundamentals of Molecular Biology (Unit II)
Mechanism of Transcription
The Process of Transcription
? Initiation
? Where/when most regulation of gene expression occurs
? Different between proks & euks
? Elongation
? Essentially same between prokaryotes & eukaryotes
? Some regulation, more in proks than euks
? Termination
? Different between proks & euks ? Some regulation
Transcription Proceeds Through 3 Steps
Transcription Unit
Initiation
Transcription factors & RNA polymerase recognize & bind the promoter
DNA adjacent to the promoter is denatured forming the open promoter complex
Elongation
RNA polymerase moves along the DNA in synthesizing a RNA transcript. Synthesis is 5'3' ? Only 1 strand of DNA is read as a template.
Termination
A termination signal is reached causing RNA polymerase to dissociated from the DNA
Prokaryotic Transcription Initiation
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