Lab exercise 3 - University of Wisconsin



Animal Science 434 - Laboratory Exercise 3 – Questions

Now consider a hen's egg. Draw a representation of what sections will appear like when cut using the planes indicated? (Don't forget the yolk!)

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Familiarize yourself with the types of epithelial tissue shown below by drawing a sketch of cuboidal, columnar, stratified squamous and psuedostratified columnar. These are the types we will encounter in reproductive tract tissues. In addition to the drawing, list at least one example of a reproductive tract tissue you observe in lab to have that type of epithelial tissue.

Male Histology:

Observe the epithelial types in the organs. How does the epithelial type contribute to the function of the organ? Complete the table below to help you identify different organs.

|Organ |Epithelial type |Function of Organ |

|Seminiferous tubule | | |

|Epidiymis | | |

|Vas deferens | | |

|Seminal vesicles | | |

|Prostate | | |

|Cowper’s gland (what | | |

|do you expect since you do not have a| | |

|slide of this one?) | | |

Do all the seminiferous tubules appear to have the same types of cells present inside them?

What distinguishes the testis from the epididymis? Sketch a picture of the testis and epididymis in the same field of view so you can remember how to distinquish them from one another.

What type of epithelium is present in the epididymis and how many tubules are present? What possible function might the steriocillia in the epididymis play?

Inside the lumen of the epididymis is a mass of sperm. What does this suggest to you about the sperm storage capacity of the epididymis?

What distinguishes the vas deferens from the testis and epididymis? Draw a sketch of the vas deferens to help you remember this. What does the extensive amount of muscle in the vas deferens indicate to you?

What is the term for cutting the vas deferens to make a male sterile?

Draw a sketch of the seminal vesicles and the prostate gland for future reference.

What are the tubules present in the cross section of the fetal testis? Within these tubules, what are the two types of cells called?

In the image of the normal dog testis, the seminiferous tubule is clearly visible and contains multiple cell types. What are the names of these different cell types?

In the image of the abnormal dog testis, the seminiferous tubules look very different than that seen in the normal dog. What types of cells are visible in these abnormal seminiferous tubules? What may have happened to this testis and how will this affect the animal?

Female Histology:

14. Ovary

a. What type of follicle was the most prevelant on the fetal pig ovary and how did this compare to the types of follicles present in the superovulated mouse or the mouse reproductive tract slides?

b. Draw a diagram of a primordial, primary, secondary and tertiary follicle to remind yourself of the differences in these follicles.

15. Oviduct

a. What is the difference in the appearance of the isthmus and ampulla of the oviduct?

b. Draw a sketch to remind yourself.

16. Cervix

a. What type of epithelium did you observe in the cervix?

b. What type of tissue is next to this epithelia?

c. What does this suggest to you about the mechanism of cervical dilation at parturition?

17. Uterus

a. What is the name of the muscularis region in the uterus and how many layers are present in this region? Why are so many layers needed in the uterus?

18. You examined an image of a recently ovulated oocyte. Where in the oviduct was the oocyte located? What were the dots within the oviduct lumen that surround the oocyte?

Leptospirosis:

19. During a chronic infection, what is common among cattle, swine, horses and sheep as to when leptospirosis causes abortion during pregnancy?

20. What should you do if your animals are diagnosed with this disease?

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