Aerobic Gram-Negative Rods Flowchart
Aerobic Gram-Negative Rods Flowchart
NOTE Exceptions do exist to these general rules
* = See Biochemical Tests for Gram Negative
Organism ID Job aid for positive and negative
Yes
result reference.
Lactose fermentation on Mac
Gram Stain = Gram Negative Rods or Gram Negative Coccobacilli
Growth on MacConkey Agar
No
Growth on Blood Agar
Lactose Fermenter
Lactose Non-fermenter
Yes
No
Indole test ? used for presumptive ID only ? Full Identification necessary
Indole (+)*
Indole (-)*
Oxidase Test ? used for presumptive ID only ? Full Identification necessary
Oxidase (+)*
Oxidase (-)*
Brucella spp., Francisella spp., Eikenella spp., Pasteurella spp., Capnocytophaga
Growth on CHOC
Probable Haemophilus
spp.
Escherichia coli,
Klebsiella oxytoca,
Citrobacter diversus,
Edwardsiella tarda
Cronobacter spp. ,
Klebsiella pneumoniae,
Enterobacter aerogenes,
Enterobacter cloacae,
Hafnia alvei
Pseudomonas spp., Alcaligenes spp., Vibrio spp., Aeromonas spp.,
Burkholderia spp.variable
Rule in/Rule out procedure for BT agents: Burkholderia mallei ? non-
motile, Burkholderia pseudomallei ? motile, Both organisms are Indole negative and Colistin
resistant
Acinetobacter spp., Stenotrophomonas
spp., Proteus spp., Yersinia spp., Salmonella spp., Shigella spp.,
B. mallei
If probable B. mallei, use rule in/rule out procedure to left
Rule in/Rule out procedure for BT
agents:
Francisella spp: Oxidase negative, Catalase negative or weakly positive
Brucella spp: Oxidase positive, Urease positive, Catalase positive
This job aid is a component of the free, on-demand CDC training course "Biochemicals and Gram Negative Organism ID." Find the course at
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