ZYMED BrdU STAINING KIT - Sites@Duke
1. Quench in 3% H2O2 in methanol 10 min. 2. PBS x 3 2 min each. 3. Trypsin .0625% in dH2O – preheat to 37oC 5 min 37o. 4. dH2O x 3 2 min each. 5. Denaturing solution (reagent 2) 100ul/slide 30 min. 6. PBS x 3 2 min. 7. Blocking solution (reagent 3) 100 ul/slide 10 min. 8. ................
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