ABC METHOD – VECTASTAIN ELITE IMMUNOPEROXIDASE KIT …
1. Methanol / 5% H2O2 – freshly made 30 minutes. 2. Running tap H2O 10 minutes. 3. Pretreatment (if required) followed by wash in H2O 4. Tris 5 minutes. 5. Tris/FBS 5 minutes. 6. Primary antibody 1 hour. 7. Tris/tween 5 minutes. 8. Tris 5 minutes. 9. Tris/FBS 5 minutes. 10. Secondary antibody 30 minutes ................
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