Immunohistochemistry Protocol - Dartmouth College
ABC solution (Vector Elite Kit) Methanol . Petri Dishes (for mounting) Small paint brush. Small ICC dishes (12 wells/dish) Day 1. Experiment done, in most cases, in room temperature. If sections are in solution other than PBS rinse sections in PBS/TX-100. Incubate for 20 min in the following solution: For 3 ml/well: 10% Methanol 300 ul. 0.1% ... ................
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