USE OF OILSEED MEALS AS FISH MEAL REPLACER IN TILAPIA …



Use of Oilseed Meals as Fish Meal Replacer in Tilapia Diets

O. A. Fagbenro* and S. J. Davies**

* Department of Fisheries and Wildlife, Federal University of Technology

P.M.B. 704 Akure, Nigeria

Alfabol@.ng

** Fish Feed Technology/Fish Nutrition Unit, Department of Biological Sciences,

University of Plymouth, Drake Circus,Plymouth, Devon PL4 8AA, UK.

ABSTRACT

Mechanically-extracted meals derived from soybean, sunflower seed, peanut, roselle seed, cottonseed, benneseed (sesame seed) and winged bean were tested individually and compared with a control diet containing fish meal for mixed-sex Oreochromis niloticus. Apparent crude protein and gross energy digestibilities of the oilseed meals were not similar (P < 0.05). The effect of replacing 67% of fish meal in the control diet with each oilseed meal on growth response, feed conversion efficiency and protein utilization was evaluated. Practical diets (240 g digestible protein/kg diet and 12.5 MJ digestible energy/ kg diet) were fed to O. niloticus fingerlings (8.1∀0.3 g) to apparent satiation twice daily for 70 days. Replacement of 67% of fish meal protein with soybean meal, sunflower seed meal or winged bean meal did not affect growth response, feed conversion efficiency or protein utilization by tilapia, and were similar (P > 0.05) to those fed with the control diet. There was growth retardation and poor feed utilization when peanut meal, benneseed meal, cottonseed meal or roselle seed meal replaced 67% of fish meal protein. The nutritive value of the different oilseed meals is discussed in relation to some essential amino acid levels and toxic or anti-nutrients.

INTRODUCTION

Fish meal, the conventional protein source in aquaculture feeds, supports good fish growth because of its protein quality and palatability. Fish meal is often scarce and expensive especially good quality brands, hence the cost of fish production and nutrition is often very high. According to Rumsey (1993) and Tacon (1993), cost-effective, practical aquaculture feeds can be produced without the use of fish meal with no resulting or apparent loss in fish growth. Hence, there is a growing demand to substitute fish meal with less expensive protein-rich plant sources (Rumsey 1993) and in this respect, legume seeds and oilseed meals have economic potentials (De la Pena et al. 1987; Lim & Dominy 1991). While grain legumes have not been widely used within aquaculture feeds, oilseeds and their by-products usually constitute a major source of dietary protein within aquaculture feeds for warmwater omnivorous/herbivorous fish species commonly used in African aquaculture, such as tilapias (Oreochromis spp.) and catfishes (Clarias spp.). Soybean products have been successfully used as partial substitutes for fish meal in diets for Mozambique tilapia, O. mossambicus (Jauncey et al. 1983; Davies et al. 1989), hybrid tilapia, O. niloticus x O. aureus (Shiau et al. 1987, 1990) and African catfish, Clarias gariepinus (Balogun & Ologhobo 1989; Sadiku & Jauncey 1994; Fagbenro & Davies 2000). Relatively, work on the use of other oilseed meals such as peanut meal, benneseed (sesame) meal, cottonseed meal, palm kernel meal or sunflower meal in warmwater fish nutrition is limited, but are known to be used effectively at low levels by O. mossambicus (Jackson et al. 1982), O. aureus (Robinson et al. 1984), O. niloticus (Ofojekwu & Ejike 1984; Balogun & Fagbenro 1985; El-Sayed 1990; Omoregie & Ogbemudia 1993), red tilapia (Hashim et al. 1994) and C. gariepinus (Olukunle & Falaye 1998). While roselle seed (Hibiscus sabdariffa) meal has been used to replace groundnut meal with no adverse effects on chick growth (Mohammed & Idris 1991; Backeit et al. 1994), it has however not been tested in fish diets.

This study evaluates the nutritive potential of various mechanically-extracted oilseed meals (soybean meal, sunflower meal, peanut meal, roselle seed meal, cottonseed meal, benneseed meal, winged bean meal) as replacement for 67% of fish meal protein in diets for fingerlings of Nile tilapia (Oreochromis niloticus).

MATERIALS AND METHODS

Menhaden fish meal, mechanically-extracted meals derived from soybean seed, sunflower seed, peanut, roselle seed, cottonseed, benneseed, winged bean seed, and other feedstuffs were separately milled, screened to fine particle size (< 250 μm), and triplicate samples were analyzed. Proximate analyses for moisture, crude protein (N x 6.25), crude lipid, crude fibre and total ash were conducted in triplicate according to AOAC (1990) methods. Lysine and methionine contents of the diets were determined from tabular values of feedstuffs (Tacon 1993). Gross energy was determined by bomb calorimetry using an adiabatic Gallenkamp calorimeter.

A purified reference diet was prepared as formulated by Fagbenro (1998). Test feedstuff diets containing 70% of the reference diet mixture and 30% of each of the oilseed meals were also formulated. Chromium III oxide was included in all diets (10 g/kg) as the inert digestibility marker. Dry extruded diets (5 mm diameter, 1 cm long) were prepared as previously described (Fagbenro 1998) and stored (-20oC) in airtight polyethylene bags until fed. Nile tilapia fingerlings were randomly distributed in groups of 15 fish into 120-litre cylindrical plastic tanks supplied with aerated water. Each diet was assigned to duplicate tanks and tilapia were fed to apparent satiation twice daily (8.30-9.00 h and 16.00-16.30 h) for 14 days. On the last day, faeces were collected from each anaesthetized tilapia (2.5 ml quinaldine/litre of water) eight hours after feeding using the dissection method (De Silva et al. 1990). Dry matter and crude protein were analyzed in triplicate samples of diets and faeces according to AOAC (1990) methods and gross energy content was determined by bomb calorimetry. Chromium content of diets and faeces was determined spectrophotometrically following the method of Bolin et al. (1952).

Apparent digestibility coefficient (ADC) of crude protein and gross energy in the test and reference diets were calculated as: ADCnutrient = 102 - [102 x (Id /If x Nf /Nd)], where: Nd = nutrient in diet, Nf = nutrient in faeces; Id = Cr2O3 in diet; If = Cr2O3 in faeces. The ADCnutrient in test feedstuff was calculated as: ADCnutrient = 100/30 (ADCtest diet - 70/100 ADCreference diet)

Based on the nutrient composition, ADCcrude protein and ADCgross energy of oilseed meals (Table 1), eight isoproteic and isocaloric (240 g digestible protein/kg diet and 12.5 MJ digestible energy/kg dry matter) pelleted diets were formulated (Table 2) to meet the nutrient requirements of Nile tilapia (Luquet 1991). A control diet (diet 1) contained menhaden fish meal as the main protein source which was replaced with each oilseed meal at 67% level in diets 2-8. Diets were formulated to contain equal animal oil. The feedstuffs were blended, moistened, cold-pelleted, oven-dried for 24 h, and stored in sealed (air-dry) plastic containers at ambient temperature (25 oC). The diets were also tested for water stability according to the method described by Fagbenro & Jauncey (1995).

Table 1. Proximate composition, energy content (MJ/kg), lysine and methionine contents (g/kg protein) and apparent digestibility coefficients (%) of oilseed meals.

| | | | | | | | |

| |Soybean |Sunflower |Peanut meal|Roselle |Benneseed |Cotton |Winged bean |

| |meal |Seed meal | |Seed meal |meal |seed |meal |

|G/kg dry wt. | | | | | |meal | |

| | | | | | | | |

|Dry matter |890 |922 |904 |926 |920 |922 |904 |

| | | | | | | | |

|Crude protein |433 |411 |458 |451 |425 |411 |420 |

| | | | | | | | |

|Crude lipid |51 |53 |63 |59 |72 |69 |55 |

| | | | | | | | |

|Crude fibre |49 |115 |55 |127 |54 |124 |65 |

| | | | | | | | |

|Total ash |58 |65 |45 |114 |96 |65 |52 |

| | | | | | | | |

|Gross energy |17.7 |17.3 |18.1 |17.9 |17.6 |17.2 |17.5 |

| | | | | | | | |

|Lysine |67 |39 |31 |41 |28 |39 |68 |

| | | | | | | | |

|Methionine |14 |27 |11 |15 |28 |15 |12 |

| | | | | | | | |

|ADC crude protein |90.2 |85.8 |91.6 |85.1 |89.4 |86.8 |90.6 |

| | | | | | | | |

|ADC gross energy |62.7 |63.6 |66.4 |62.5 |65.3 |65.0 |64.1 |

Groups of 20 tilapia fingerlings (mean weight, 8.1∀0.3 g) were acclimated to experimental conditions for 14 days and randomly stocked into an indoor recirculating system comprising plastic tanks provided with continuous aeration. Each diet was fed to tilapia in duplicate tanks (in a randomized design), to apparent satiation twice daily (09.00 h, 16.00 h) for 70 days. Individual fish in each tank were weighed at the start and every 14 days to monitor growth response and feed utilization according to Steffens (1989). At the end of the feeding trial, five tilapia were randomly selected from each tank, dissected and the livers were removed, weighed and used to estimate the hepatosomatic index (HSI).

The data obtained were subjected to one-way analysis of variance (ANOVA) test, where P < 0.05 was judged indicative of a significant difference. Where the ANOVA revealed significant differences, Duncan's multiple-range test was applied to characterize and quantify the differences between treatments using Statgraphics 3.1 Plus package for Windows on IBM PC (Statistical Graphics Corp, US).

Table 2. Formulation (g/kg dry matter) and nutrient composition of diets.

| | | | | | | | | |

| |1 |2 |3 |4 |5 |6 |7 |8 |

| | | | | | | | | |

|Menhaden fish meal |400 |100 |100 |100 |100 |100 |100 |100 |

| | | | | | | | | |

|Soybean meal |- |464 |- |- |- |- |- |- |

| | | | | | | | | |

|Sunflower meal |- |- |514 |- |- |- |- |- |

| | | | | | | | | |

|Peanut meal |- |- |- |431 |- |- |- |- |

| | | | | | | | | |

|Roselle seed meal |- |- |- |- |471 |- |- |- |

| | | | | | | | | |

|Benneseed meal |- |- |- |- |- |476 |- |- |

| | | | | | | | | |

|Cottonseed meal |- |- |- |- |- |- |507 |- |

| | | | | | | | | |

|Macadamia nut meal |- |- |- |- |- |- |- |- |

| | | | | | | | | |

|Winged bean meal |- |- |- |- |- |- |- |475 |

| | | | | | | | | |

|Corn starch |530 |334 |283 |382 |326 |328 |296 |321 |

| | | | | | | | | |

|Cod liver oil |- |30 |30 |30 |30 |30 |30 |30 |

| | | | | | | | | |

|Corn oil |20 |22 |23 |7 |23 |16 |17 |24 |

| | | | | | | | | |

|Mineral-vitamin mixture 1 |30 |30 |30 |30 |30 |30 |30 |30 |

| | | | | | | | | |

|Carboxymethyl cellulose |20 |20 |20 |20 |20 |20 |20 |20 |

| | | | | | | | | |

|Digestible protein (g/kg) |240.1 |241.3 |241.3 |240.9 |240.8 |240.9 |240.9 |240.8 |

| | | | | | | | | |

|Digestible energy (MJ/kg) |12.6 |12.7 |12.6 |12.7 |12.7 |12.6 |12.7 |12.7 |

| | | | | | | | | |

|Water stability (% LDM) |4.4 |4.3 |4.5 |4.4 |4.6 |4.5 |4.2 |4.6 |

| | | | | | | | | |

|Lysine (g/kg protein) |29.2 |38.4 |27.3 |20.7 |26.6 |20.6 |27.1 |39.6 |

| | | | | | | | | |

|Methionine (g/kg protein) |10.8 |9.2 |16.6 |7.4 |9.8 |16.0 |10.3 |8.4 |

1 Fish pre-mix. Colborne Dawes Nutrition Ltd., UK. g/kg diet: vitamin A, 1600 IU; vitamin D, 2400 IU; vitamin E, 160 mg; vitamin K, 16 mg; thiamin, 36 mg; riboflavin, 48 mg; pyridoxine, 24 mg; niacin, 288 mg; panthotenic acid, 96 mg; folic acid, 8 mg; biotin, 1.3 mg; cyanocobalamin, 48 mg; ascorbic acid, 720 mg; choline chloride, 320 mg; calcium 5.2 g; cobalt, 3.2 mg; iodine, 4.8 mg; copper, 8 mg; iron, 32 mg; manganese, 76 mg; zinc, 160 mg; Endox (antioxidant) 200 mg.

RESULTS

The proximate composition of the oilseed meals and menhaden fish meal as well as their ADCs for dry matter, crude protein and gross energy content are presented in Table 1. ADCcrude protein of the oilseed meals ranged from 85.1% in roselle seed to 91.6% in peanut meal while ADCgross energy ranged from 56.2% in roselle seed meal to 66.4% in peanut meal, and showed no significant differences (P > 0.05) (Table 1). No differences occurred in water stability of pellets (Table 2). No tilapia mortality was recorded in all treatments, and apparent voluntary feed intakes were different (P < 0.05) among the diet treatments (Table 3). Growth response, feed efficiency and protein utilization by tilapia are also presented in Table 3 which shows that the best overall growth response was obtained in tilapia fed with the control diet. No significant differences occurred in weight gain and growth response by tilapia fed diets containing soybean meal, sunflower seed meal or winged bean meal as replacement for 67% of fish meal protein. Feed utilization indices followed a similar pattern as the growth in the treatments (Table 3). However, growth response and feed utilization was reduced in tilapia fed peanut meal, benneseed meal, cottonseed meal or roselle seed meal-based diets. HSI values did not show any trend relating to diet-treatment.

Table 3. Growth response and feed utilization by tilapia fed diets containing various oilseed meals for 70 days.

| | | | | | | | | |

|Diet |Mean final wt.1 (g) |WG2 |VFI 3 |SGR4 |FGR5 |PER6 |PPV7 |HSI8 |

| | |(%) | |(%/day) | | |(%) |(%) |

| | | | | | | | | |

|1 |32.7a |303.7a |29.44 |1.99a |1.66a |1.79a |38.59a |1.65a |

| | | | | | | | | |

|2 |31.0a |282.7a |28.09 |1.92a |1.68a |1.59a |36.65a |1.70a |

| | | | | | | | | |

|3 |30.3a |274.1b |27.71 |1.88ab |1.72a |1.22b |36.92a |1.76a |

| | | | | | | | | |

|4 |25.4b |213.6c |28.73 |1.63c |2.12b |0.90bc |30.99b |1.67a |

| | | | | | | | | |

|5 |25.7b |217.3c |26.98 |1.65bc |1.99b |0.97b |31.47b |1.66a |

| | | | | | | | | |

|6 |28.4b |250.6b |28.45 |1.79b |1.87b |1.19b |35.37a |1.70a |

| | | | | | | | | |

|7 |26.2b |223.5c |26.57 |1.68b |1.95b |1.11b |32.01b |1.65a |

| | | | | | | | | |

|8 |30.7a |279.0ab |28.89 |1.90a |1.69a |1.47a |36.78a |1.73a |

1 Mean initial fish weight = 8.1 g

2 weight gain = (final weight - initial weight/initial weight) x 100

3 voluntary feed intake = (g/kg) body weight of fish /day

4 specific growth rate = [(ln final wt. - ln initial wt.)/no of days] x 100

5 feed gain ratio = feed intake (g)/weight gain (g)

6 protein efficiency ratio = body weight gain (g)/protein fed (g)

7 protein productive value =[protein gain (g)/protein fed (g)] x 100

8 Hepatosomatic index = (liver weight/body weight) x 100

Values in a column with dissimilar letters are significantly different (P < 0.05).

DISCUSSION

According to Jauncey (1993), the most important characteristic of feedstuffs is the bioavailablity of nutrients, particularly digestible protein and digestible energy. Hence reliable data on the digestibility of different ingredients for each species might well be considered as a necessary prerequisite. Differences (P > 0.05) in apparent digestibility coefficients of crude protein and gross energy content of oilseed meals (Table 1) may result from relative quantities and chemical characteristics in their carbohydrate content. Specifically, lower protein and energy digestibility in sunflower meal has been attributed to the high crude fibre content (Sanz et al. 1994). Previous digestibility studies involving tilapias were limited to O. niloticus and were limited to mechanically-extracted soybean meal (Popma 1982; De Silva & Perera 1984; Hanley 1987; Kamarudin et al. 1989; Hossain et al. 1992; Fagbenro 1998), the results of which agree with ADCcrude protein and ADCgross energy of soybean meal used in this study. Generally, ADC crude protein and ADCgross energy of winged bean meal (Table 1) agreed with the respective values reported for full-fat winged bean meal for Nile tilapia (Fagbenro 1998) in which a similar digestibility method was used. ADCcrude protein of peanut meal and benneseed meal in this study are similar to those reported by De Silva & Perera (1984) and Hossain et al. (1992), respectively.

It is expected that growth of tilapia fed diets containing similar levels of digestible protein and digestible energy should be identical. Growth retardation and poor protein utilization was however observed in the diet treatment involving inclusion of peanut meal, benneseed meal, cottonseed meal, roselle seed meal or macadamia nut meal replacing 67% of fish meal protein. Reduced growth response and feed utilization in various warmwater aquaculture species fed diets in which fish meal component was replaced with oilseed meals have been explained by sub-optimal amino acid balance, inadequate levels of phosphorus, inadequate levels of energy, low feed intake caused by palatability, presence of endogenous anti-nutrients or dietary level of fish oil (Lim & Dominy 1991). Lower growth at 67% fish meal replacement with peanut meal, benneseed meal, cottonseed meal or roselle seed meal in this study may have been caused by one or some of these factors.

According to Liener (1975), oilseed meals contain many thermolabile anti-nutrients, most importantly enzyme inhibitors and haemagglutinins. Whether these other anti-nutrients factors were totally inactivated was not determined in this study. Since all diets were isocaloric, the problem of low digestible energy value of oilseed meals may not be relevant. The phosphorus requirements for tilapia was met in the diets (Luquet 1991). As tilapia in all treatments readily accepted the diets with similar voluntary feed intake values (Table 3), palatability did not seem to pose a problem. These then leave inadequate amino acid as the possible cause for the poor tilapia growth. Although the calculated methionine content of the diets containing soybean meal, peanut meal, roselle seed meal, cottonseed meal and winged bean meal were lower than methionine content of the control diet (Table 2), the differences may be spared by cystine. Calculated lysine level in the diets (Table 2) indicated that except for peanut and benneseed meal-based diets, lysine content of the other diets were similar to or higher than that of the control diet. Even then, in roselle seed meal-based diet or cottonseed meal-based diet where their lysine contents were similar to that of the control diet, the biological value of lysine from such diets may be less than indicated, hence the low (P > 0.05) rate of weight gain by tilapia.

Shiau et al. (1987) demonstrated that fish meal can be partially replaced by soybean meal in hybrid tilapia (O. niloticus x O. aureus) when the dietary protein level is below optimum level for growth (240 g/kg). At the optimum, level of dietary protein (320 g/kg), replacement of 30% fish meal with soybean meal significantly depressed growth and feed efficiency. However, these were restored by addition of methionine to the level of the control diet. Tacon et al. (1983) reported that supplementation of 8 g/kg DL-methionine to a diet in which 75% of brown fish meal was replaced by soybean meal improved the growth performance of O. niloticus to a level comparable to that of a fish meal diet. Viola & Arieli (1983) reported that soybean meal can be used to replace up to half of the fish meal in tilapia feeds having 250 g/kg crude protein content without requiring any supplementation. Jackson et al. (1982) also observed a growth reduction of O. mossambicus fed a diet in which 50% or more of fish meal was replaced by soybean meal. They attributed this to the low level of sulphur amino acids and the presence of endogenous anti-nutrients such as trypsin inhibitor or haemagglutinins.

Winged bean plant (Psophocarpus tetragonolobus) is indigenous to the humid tropics and all parts of the plant are consumed by man, particularly the seeds. The seeds contain remarkably high protein and oil levels and an excellent amino acid profile, and have been used in diets fed to rats (Jaffe & Korte 1976) and poultry (De Lumen et al. 1982). Hashim et al. (1994) reported the inability of winged bean meal to replace fish meal totally in a red tilapia (O. niloticus) fry diet, causing adverse effects on overall growth performance and feed utilization.

Sunflower seed meal has been reported to contain a lot of endogenous anti-nutritional factors, such as a protease inhibitor, an arginase inhibitor and the polyphenolic tannin chlorogenic acid (Tacon et al. 1984). It has relatively high crude fibre content, which can reduce the pelleting quality and protein digestibility of the feed if included at high levels. Similarly, the high crude fibre content of palm kernel meal and copra meal reduced digestibility in diets fed to Nile tilapia (Kamarudin et al. 1992; Omoregie & Ogbemudia 1993). Sunflower seed meal also contains low levels of lysine. Despite these drawbacks, sunflower meal has been reported to be a good protein source for O. mossambicus even at 696 g/kg of the diet (Jackson et al. 1982).

Peanut meal is highly palatable and has better binding properties for pelleting than soybean meal and presently, its use is limited because of low lysine and methionine contents. Peanut meal can replace 25% of fish meal in the diet of O. mossambicus without affecting growth performance. At higher levels, growth rate decreased rapidly, probably because of its low methionine content (Jackson et al. 1982).

Although cottonseed is an important source of dietary protein for domestic animals, its use in commercial aquaculture feeds is limited because of the presence of gossypol and the low available lysine content. The level of inclusion in fish diets depends mainly on the gossypol content of the meal used. Low gossypol cottonseed meal (0.03%) was reported to be a good protein source for O. mossambicus. The growth of tilapia was improved when 50% of fish meal was replaced by cottonseed meal. The growth rate was essentially the same as the control even at 100% substitution level (Jackson et al. 1982). In contrast, Ofojekwu & Ejike (1984) reported a much lower weight gain and feed efficiency of O. niloticus fed a cottonseed meal diet as compared to the tilapia fed a fish meal control diet. Dietary inclusion levels of 20-30% cottonseed meal have been reported to be both safe and useful (Robinson et al. 1984; El-Sayed 1990).

CONCLUSIONS

There is economic justification for using oilseed meals as partial replacement for fish meal in diets of Nile tilapia. The amount of oilseed used depends on the species, availability, cost, acceptability, and presence of toxins or anti-nutrients. Soybean meal, sunflower meal or winged bean meal had very similar nutrient digestibility coefficients and comparable lysine and methionine contents to those of menhaden fish meal, hence they replaced 67% of menhaden fish meal in practical diets for Nile tilapia diets without compromising fish growth and nutrient utilization. Peanut meal, benneseed meal, cottonseed meal or roselle seed meal cannot substitute 67% of menhaden fish meal in diets for Nile tilapia as they did not have similar nutritive value for incorporation in practical diets for tilapia.

ACKNOWLEDGMENTS

The Royal Society, London funded the study under the Third World Fellowship Scheme.

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